Differentiation and functional maturation of bone marrow-derived intestinal epithelial T cells expressing membrane T cell receptor in athymic radiation chimeras
- Univ. of Tulsa, OK (USA)
The thymus dependency of murine intestinal intraepithelial lymphocytes (IEL) was studied in an athymic F1----parent radiation chimera model. IEL, although not splenic or lymph node lymphocytes, from athymic chimeras displayed normal levels of cells bearing the class-specific T cell Ag, CD4 and CD8; the TCR-associated molecule, CD3; and the Thy-1 Ag. Moreover, two-color flow cytometric analyses of IEL from athymic mice demonstrated regulated expression of T cell Ag characteristic of IEL subset populations from thymus-bearing mice. In immunoprecipitation experiments, surface TCR-alpha beta or TCR-gamma delta were expressed on IEL, although not on splenic lymphocytes, from athymic chimeras. That IEL from athymic chimeras constituted a population of functionally mature effector cells activated in situ, similar to IEL from thymus-bearing mice, was demonstrated by the presence of CD3-mediated lytic activity of athymic lethally irradiated bone marrow reconstituted IEL. These data provide compelling evidence that intestinal T cells do not require thymic influence for maturation and development, and demonstrate that the microenvironment of the intestinal epithelium is uniquely adapted to regulate IEL differentiation.
- OSTI ID:
- 6789790
- Journal Information:
- Journal of Immunology; (USA), Journal Name: Journal of Immunology; (USA) Vol. 145:5; ISSN JOIMA; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CELL DIFFERENTIATION
CELL FLOW SYSTEMS
CHIMERAS
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
EPITHELIUM
GASTROINTESTINAL TRACT
INTESTINES
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MEDICINE
MICE
MOSAICISM
ORGANS
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
SURGERY
THYMECTOMY
THYMUS
TISSUES
TRANSPLANTS
VERTEBRATES