A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase
Journal Article
·
· Genomics; (United States)
- Univ. of Western Ontario (Canada)
- London Regional Cancer Centre, Ontario (Canada)
A cDNA clone was isolated from HL60 human promyelocyte cells on the basis of the induction of its cognate mRNA by the phorbol ester TPA (12-O-tetrade-canoyl phorbol-13-acetate) in the presence of cycloheximide. Northern analysis showed a peak of induction of a 1.9-kb mRNA at 3-6 h after TPA addition. Sequence analysis revealed that the cDNA was likely a human homolog of yeast isopentenyl diphosphate:dimethylallyl diphosphate isomerase, an enzyme important for isoprenoid synthesis and the prenylation of proteins such as ras p21. 14 refs., 2 figs.
- OSTI ID:
- 6789721
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 20:1; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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