Isolation and characterization of human glycophorin A cDNAs using a synthetic oligonucleotide approach: nucleotide sequence, mRNA structure and regulation by 12-O-tetradecanoylphorbol 13-acetate (TPA)
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5116978
The authors have previously shown that treatment of human erythroleukemic K562 cells with the tumor-promoting phorbol ester, TPA, results in a diminished expression of glycophorin A at the level of protein biosynthesis and in vitro mRNA translation activity. To further examine the structure, relationships and expression of human glycophorins they have successfully isolated and sequenced several glycophorin A specific cDNA clones derived from K562 cells, by making extensive use of mixed and exact synthetic oligonucleotides as primers and radioactively labeled probes. The nucleotide sequence obtained from the largest glycophorin A cDNA suggests the presence of a hydrophobic leader-like peptide of at least 19 amino acids. Northern gel analysis using both whole cDNA-plasmid and synthetic oligonucleotide probes revealed the existence of multiple mRNAs, three of which they believe to be glycophorin A-specific, whereas a fourth and smaller mRNA appears to be glycophorin B-specific. Furthermore, the abundance of all four glycophorin mRNAs were found to be extensively reduced following treatment of K562 cells with TPA suggesting coordinate regulation, possibly at the level of gene transcription.
- Research Organization:
- La Jolla Cancer Research Foundation, CA
- OSTI ID:
- 5116978
- Report Number(s):
- CONF-8606151-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
- Country of Publication:
- United States
- Language:
- English
Similar Records
Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure
Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A
A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase
Journal Article
·
Fri Feb 28 23:00:00 EST 1986
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:5879014
Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A
Journal Article
·
Thu Oct 01 00:00:00 EDT 1987
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
·
OSTI ID:7242242
A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase
Journal Article
·
Mon Feb 28 23:00:00 EST 1994
· Genomics; (United States)
·
OSTI ID:6789721
Related Subjects
550200 -- Biochemistry
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CHEMICAL COMPOSITION
DISEASES
DNA
ERYTHROCYTES
ESTERS
GLUCOPROTEINS
HEMIC DISEASES
LEUKEMIA
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PRIMATES
PROTEINS
PURIFICATION
RNA
SACCHARIDES
TUMOR CELLS
VERTEBRATES
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
CHEMICAL COMPOSITION
DISEASES
DNA
ERYTHROCYTES
ESTERS
GLUCOPROTEINS
HEMIC DISEASES
LEUKEMIA
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PRIMATES
PROTEINS
PURIFICATION
RNA
SACCHARIDES
TUMOR CELLS
VERTEBRATES