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Stimulation of proliferation of HL60 cells by low concentrations of 12-O-tetradecanoylphorbol-13-acetate and its relationship to the mitogenic effects of insulin

Journal Article · · Experimental Cell Research; (United States)
;  [1]
  1. St. George's Hospital Medical School, London (United Kingdom)
+ Show Author Affiliations

The effects of the phorbol ester 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the growth and differentiation of cultured human acute promyelocytic leukemia (HL60) cells have been studied using cells growing in a fully defined medium consisting of RPMI 1640 supplemented with selenium dioxide, insulin, and either transferrin or ferric citrate. High concentrations of TPA cause the expected inhibition of proliferation and induction of macrophage-like differentiation. In contrast, in cells deprived in insulin, which continue to grow at a slow rate, lower concentrations of TPA stimulate proliferation without inducing differentiation. The ability of higher concentrations of TPA to induce differentiation is independent of the presence of insulin. Low-TPA also stimulates the short-term incorporation of thymidine by three- to fourfold, as compared to a seven fold stimulation by insulin. The proliferative response to low TPA concentrations provides a useful model for dissecting the signaling pathways that control cell proliferation following stimulation by insulin and activators of protein kinase C.

OSTI ID:
6969007
Journal Information:
Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 199:1; ISSN 0014-4827; ISSN ECREAL
Country of Publication:
United States
Language:
English

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Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CARCINOGENS
CELL PROLIFERATION
CHALCOGENIDES
DISEASES
ENZYMES
ESTERS
GLOBULINS
GLOBULINS-BETA
HORMONES
IMMUNE SYSTEM DISEASES
INSULIN
LEUKEMIA
METALLOPROTEINS
NEOPLASMS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
SELENIUM COMPOUNDS
SELENIUM OXIDES
TRANSFERASES
TRANSFERRIN
TUMOR CELLS