Optimized fluorometric determination of urinary delta-aminolevulinic acid by using pre-column derivatization, and identification of the derivative
- Shiga Univ. of Medical Science (Japan)
We previously reported the usefulness of a fluorometric method to determine urinary delta-aminolevulinic acid (ALA) concentrations by using post-column derivatization to monitor the effect of lead exposure. We have further improved the method by introducing pre-column derivatization by using reaction of ALA with acetylacetone and formaldehyde. Response of the hematopoietic system to lead exposure can now be easily detected at blood lead concentrations as low as 162 micrograms/L. The fluorescent ALA derivative, a new aromatic product, 2-methylideneamino-3,5-diacetyl-4,6-dimethylphenylpropionic acid, is separated on octadecyl silica column by high-performance liquid chromatography and the fluorescence intensity is detected with a fluorophotometer. Sample recoveries for 12 urine samples from workers exposed to lead and unexposed controls were 91.9-110.2%. The results obtained by the pre-column derivatization method agreed with those by the post-column derivatization method. The new method increases the sensitivity to a detection limit to 10 micrograms of delta-aminolevulinic acid per milliliter of urine and is simple enough to be used for routine monitoring of the biological effect of exposure to low concentrations of lead.
- OSTI ID:
- 6788515
- Journal Information:
- Clinical Chemistry (Winston-Salem, North Carolina); (USA), Journal Name: Clinical Chemistry (Winston-Salem, North Carolina); (USA) Vol. 36:8 Pt 1; ISSN 0009-9147; ISSN CLCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
AMINOLEVULINIC ACID
ANIMALS
BIOLOGICAL INDICATORS
BIOSYNTHESIS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ELEMENTS
FLUORESCENCE
LEAD
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
MAMMALS
MAN
MASS SPECTRA
MEASURING METHODS
METABOLISM
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
SENSITIVITY ANALYSIS
SEPARATION PROCESSES
SPECTRA
SYNTHESIS
VERTEBRATES