Effect of paraquat on microsomal lipid peroxidation in vitro and in vivo. [Rats, rabbits, man, mice]

Journal Article · Tue Jan 01 00:00:00 EST 1980 · Toxicol. Appl. Pharmacol.; (United States)

DOI:https://doi.org/10.1016/0041-008X(80)90433-0· OSTI ID:6786674

Kornbrust, D J; Mavis, R D

Rat lung and liver microsomes did not undergo lipid peroxidation in the absence of iron when incubated with NADPH and concentrations of paraquat ranging from 10/sup -7/ to 10/sup -2/ M. Paraquat also did not stimulate rat liver and lung microsomal peroxidation induced by added iron and NADPH, and was inhibitory at concentrations above 10 ..mu..M. Similarly, no stimulation of peroxidation was produced by paraquat in rabbit or human lung microsomes; however, under similar conditions, paraquat enhanced NADPH/iron-dependent peroxidation in mouse lung and liver microsomes obtained from rats sacrificed at 12, 18, and 24 hr following a lethal dose of paraquat (50 mg/kg, ip), there was no loss of vitamin E or increase in susceptibility to in vitro peroxidation which would be expected if lipid peroxidation had occurred in vivo although extensive lung damage developed during this time period. These results indicate that paraquat does not cause pulmonary toxicity by initiating peroxidation of lung lipids, and the decrease of palmitate in paraquat-damaged lungs is consistent with inhibition of fatty acid synthesis as an early event in the pathogenesis of paraquat toxicity.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6786674
• Journal Information: Toxicol. Appl. Pharmacol.; (United States), Vol. 53
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HERBICIDES
SIDE EFFECTS
HOMOGENATES
ENZYME ACTIVITY
LIPIDS
PEROXIDASES
LIVER
BIOLOGICAL EFFECTS
LUNGS
MICROSOMES
HEXADECANOIC ACID
IN VITRO
IN VIVO
IRON
MAN
MICE
NADP
RABBITS
RATS
VITAMIN E
ANIMALS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
COENZYMES
DIGESTIVE SYSTEM
ELEMENTS
ENZYMES
GLANDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
METALS
MONOCARBOXYLIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PESTICIDES
PORPHYRINS
PRIMATES
RESPIRATORY SYSTEM
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
VITAMINS
550200* - Biochemistry