Microsomal lipid peroxidation as a mechanism of cellular damage. [Dissertation]
The NADPH/iron-dependent peroxidation of lipids in rat liver microsomes was found to be dependent on the presence of free ferrous ion and maintains iron in the reduced Fe/sup 2 +/ state. Chelation of iron by EDTA inhibited peroxidation. Addition of iron, after preincubation of microsomes in the absence of iron, did not enhance the rate of peroxidation suggesting that iron acts by initiating peroxidative decomposition of membrane lipids rather than by catalyzing the breakdown of pre-formed hydroperoxides. Liposomes also underwent peroxidation in the presence of ferrous iron at a rate comparable to intact microsomes and was stimulated by ascorbate. Carbon tetrachloride initiated lipid peroxidation in the absence of free metal ions. Rates of in vitro lipid peroxidation of microsomes and homogenates were found to vary widely between different tissues and species. The effects of paraquat on lipid peroxidation was also studied. (DC)
- Research Organization:
- Rochester Univ., NY (USA). Dept. of Radiation Biology and Biophysics
- DOE Contract Number:
- EY-76-C-02-3490
- OSTI ID:
- 5825958
- Report Number(s):
- UR-3490-1765
- Resource Relation:
- Other Information: Thesis
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPIDS
METABOLISM
MICROSOMES
ASCORBIC ACID
BIOLOGICAL PATHWAYS
BIOLOGICAL VARIABILITY
CARBON TETRACHLORIDE
EDTA
ESTERS
IRON
LIVER
NADP
PEROXIDASES
PEROXIDES
RATS
RESPONSE MODIFYING FACTORS
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHELATING AGENTS
CHLORINATED ALIPHATIC HYDROCARBONS
COENZYMES
DIGESTIVE SYSTEM
ELEMENTS
ENZYMES
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
MAMMALS
METALS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PORPHYRINS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
VITAMINS
550200* - Biochemistry