Microsomal lipid peroxidation as a mechanism of cellular damage. [Dissertation]

Technical Report · Mon Jan 01 04:00:00 EST 1979

DOI:https://doi.org/10.2172/5825958· OSTI ID:5825958

Kornbrust, D. J.

The NADPH/iron-dependent peroxidation of lipids in rat liver microsomes was found to be dependent on the presence of free ferrous ion and maintains iron in the reduced Fe/sup 2 +/ state. Chelation of iron by EDTA inhibited peroxidation. Addition of iron, after preincubation of microsomes in the absence of iron, did not enhance the rate of peroxidation suggesting that iron acts by initiating peroxidative decomposition of membrane lipids rather than by catalyzing the breakdown of pre-formed hydroperoxides. Liposomes also underwent peroxidation in the presence of ferrous iron at a rate comparable to intact microsomes and was stimulated by ascorbate. Carbon tetrachloride initiated lipid peroxidation in the absence of free metal ions. Rates of in vitro lipid peroxidation of microsomes and homogenates were found to vary widely between different tissues and species. The effects of paraquat on lipid peroxidation was also studied. (DC)

• Research Organization: Rochester Univ., NY (USA). Dept. of Radiation Biology and Biophysics
• DOE Contract Number: EY-76-C-02-3490
• OSTI ID: 5825958
• Report Number(s): UR-3490-1765
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ASCORBIC ACID
BIOLOGICAL PATHWAYS
BIOLOGICAL VARIABILITY
BODY
CARBON TETRACHLORIDE
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHELATING AGENTS
CHLORINATED ALIPHATIC HYDROCARBONS
COENZYMES
DIGESTIVE SYSTEM
EDTA
ELEMENTS
ENZYMES
ESTERS
GLANDS
HALOGENATED ALIPHATIC HYDROCARBONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IRON
LIPIDS
LIVER
MAMMALS
METABOLISM
METALS
MICROSOMES
NADP
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
PEROXIDES
PORPHYRINS
RATS
RESPONSE MODIFYING FACTORS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
VITAMINS