Crocidolite-induced lipid peroxidation in rat lung microsomes. I. Role of different ions

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Environ. Res.; (United States)

OSTI ID:5714883

Gulumian, M; Kilroe-Smith, T A

Lipid peroxidation is increased by ferrous or ferric ions in rat lung microsomes both from rats pretreated with 3-methylcholanthrene and from untreated controls. This increase was dependent on the concentration of these ions in the reaction mixture. Crocidolite alone increased peroxidation in microsomal fractions. However, addition of ferric or ferrous ions with the crocidolite did not give a greater increase in the amount of peroxidation in microsomes. Chelation with EDTA or iron, whether originally present as free ions in the solution or attached to crocidolite, prevented lipid peroxidation. NADPH alone, when added to the microsomal fractions, did not produce any significant effect. However, when added concomitantly with crocidolite fibers, NADPH reduced the effect of crocidolite on lipid peroxidation. Magnesium, manganese, and calcium ions did not produce any significant effect on lipid peroxidation in the presence or absence of crocidolite. A reduced pH enhanced the rate of lipid peroxidation in line with increase solubility of iron salts at these pH values. All the above observations, taken together, lead to the conclusion that it is the iron in the crocidolite that is responsible for the latter's ability to enhance lipid peroxidation.

• Research Organization: National Centre for Occupational Health, Johannesburg, South Africa
• OSTI ID: 5714883
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 43:1; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
AMINO ACIDS
ANIMALS
AROMATICS
ASBESTOS
BIOLOGICAL EFFECTS
BODY
CALCIUM
CARBOXYLIC ACIDS
CATIONS
CELL CONSTITUENTS
CHARGED PARTICLES
CHELATING AGENTS
CHEMICAL REACTIONS
EDTA
ELEMENTS
HYDROCARBONS
IONS
IRON COMPOUNDS
LIPIDS
LUNGS
MAGNESIUM
MAMMALS
MANGANESE
METALS
MICROSOMES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDATION
POLYCYCLIC AROMATIC HYDROCARBONS
RATS
RESPIRATORY SYSTEM
RODENTS
TOXICITY
TRANSITION ELEMENT COMPOUNDS
TRANSITION ELEMENTS
VERTEBRATES