Localization of low density lipoproteins in vivo: Final technical report, September 28, 1987--September 27, 1988

Technical Report · Thu Dec 01 04:00:00 EST 1988

OSTI ID:6785105

Harper, P V

We have found that low density lipoprotein derivatized with I-123- tyramine cellobiose (I-123-TyC-LDL) behaves like native LDL when used for biodistribution studies in animals and in humans, and that I-123-TyC-LDL appears more promising than Tc-99m-labeled LDL for nuclear imaging of the coronary circulation. Unfortunately, isolation of LDL for direct derivatization with I-123-TyC is both time-consuming and affords a low but real probability of contamination. Methods for radiolabelling LDL indirectly or without prior purification would circumvent this problem. Moreover, modifications that could increase the specific activity of I-123-labelled LDL are desirable. For this project we proposed to establish a novel, rapid method for indirect radioiodination of LDL particles to be used in experimental and clinical nuclear imaging studies. We have found that small, model beta-strand peptides (MBPs) very selectively bind to LDL and that MBPs can be labelled with I-123-TyC. We have analyzed the in vivo behavior of two different types of semisynthetic I-123-TyC-LDL particles/endash/generated by adsorption of radiolabelled MBPs to native LDL/endash/in rabbits. With either preparation, clearance of radiolabelled MBPs from the circulation was more rapid than that of native LDL. 6 refs., 6 figs.

• Research Organization: Chicago Univ., IL (USA)
• DOE Contract Number: FG02-87ER60614
• OSTI ID: 6785105
• Report Number(s): DOE/ER/60614-1; ON: DE89005749
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400700 -- Radiochemistry & Nuclear Chemistry
550600 -- Medicine
560160* -- Radionuclide Effects
Kinetics
& Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARDIOVASCULAR SYSTEM
CELLOBIOSE
CHEMICAL REACTIONS
DISACCHARIDES
DOCUMENT TYPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
HEART
HOURS LIVING RADIOISOTOPES
IMAGE PROCESSING
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 123
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIPIDS
LIPOPROTEINS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PROCESSING
PRODUCTION
PROGRESS REPORT
PROTEINS
RABBITS
RADIOISOTOPES
RADIOPHARMACEUTICALS
SACCHARIDES
VERTEBRATES