Influence of Ca/sup 2 +/ concentration on phosphate metabolism of isolated rat hearts investigated with /sup 31/P NMR spectroscopy

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6782801

Hannigan, J J; Omachi, A; Labotka, R J

Calcium ions are intimately involved in the production of myocardial damage during experimental cardioplegia and reperfusion. In the present study, isolated rat (Langendorff) hearts were perfused with Krebs-Henseleit (KH) medium containing 0.25, 1 or 3 mM Ca/sup 2 +/ at 34/sup 0/. Midway during a 45 min period of K/sup +/ cardioplegia, no phosphocreatine (PC) was evident but immediately after reperfusion with KH medium, PC increased and ultimately reached 85% of the original values in 20-40 min. The ..beta..-P of ATP decreased during ischemia but showed no further change during reperfusion, the final values being 15% of the original values with 3 mM of 60% with lower Ca/sup 2 +/ levels (0.25 and 1 mM). P/sub i/ values appeared to plateau during ischemia except for the final point with 3 mM Ca/sup 2 +/ when P/sub i/ was abruptly higher. This appeared to correspond with a sharp decrease in total adenine nucleotide. During reperfusion, P/sub i/ levels declined to near preischemic values with the two lower Ca/sup 2 +/ groups but remained elevated in the 3 mM Ca/sup 2 +/ group. The total P at the end of the experiments averaged 95% of the original values for all groups indicating that there was no significant loss of P during the course of these studies. Studies with the normothermic Langendorff preparation may clearly reveal the influence of Ca/sup 2 +/ in the metabolic disturbances seen during experimental cardioplegia and reperfusion.

• Research Organization: Univ. of Illinois College of Medicine, Chicago
• OSTI ID: 6782801
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM COMPOUNDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DISEASES
HEART
ISCHEMIA
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
METABOLISM
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXYGEN COMPOUNDS
PERFUSED ORGANS
PHOSPHATES
PHOSPHORUS 31
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RATS
REACTION KINETICS
RESONANCE
RODENTS
SPECTRA
STABLE ISOTOPES
VASCULAR DISEASES
VERTEBRATES