Influence of Ca/sup 2 +/ concentration on phosphate metabolism of isolated rat hearts investigated with /sup 31/P NMR spectroscopy

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6782801

Hannigan, J J; Omachi, A; Labotka, R J

Calcium ions are intimately involved in the production of myocardial damage during experimental cardioplegia and reperfusion. In the present study, isolated rat (Langendorff) hearts were perfused with Krebs-Henseleit (KH) medium containing 0.25, 1 or 3 mM Ca/sup 2 +/ at 34/sup 0/. Midway during a 45 min period of K/sup +/ cardioplegia, no phosphocreatine (PC) was evident but immediately after reperfusion with KH medium, PC increased and ultimately reached 85% of the original values in 20-40 min. The ..beta..-P of ATP decreased during ischemia but showed no further change during reperfusion, the final values being 15% of the original values with 3 mM of 60% with lower Ca/sup 2 +/ levels (0.25 and 1 mM). P/sub i/ values appeared to plateau during ischemia except for the final point with 3 mM Ca/sup 2 +/ when P/sub i/ was abruptly higher. This appeared to correspond with a sharp decrease in total adenine nucleotide. During reperfusion, P/sub i/ levels declined to near preischemic values with the two lower Ca/sup 2 +/ groups but remained elevated in the 3 mM Ca/sup 2 +/ group. The total P at the end of the experiments averaged 95% of the original values for all groups indicating that there was no significant loss of P during the course of these studies. Studies with the normothermic Langendorff preparation may clearly reveal the influence of Ca/sup 2 +/ in the metabolic disturbances seen during experimental cardioplegia and reperfusion.

• Research Organization: Univ. of Illinois College of Medicine, Chicago
• OSTI ID: 6782801
• Report Number(s): CONF-8604222-; TRN: 87-008697
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
CALCIUM COMPOUNDS
BIOCHEMICAL REACTION KINETICS
HEART
ISCHEMIA
PHOSPHATES
METABOLISM
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PERFUSED ORGANS
PHOSPHORUS 31
RATS
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BODY
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DISEASES
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
REACTION KINETICS
RESONANCE
RODENTS
SPECTRA
STABLE ISOTOPES
VASCULAR DISEASES
VERTEBRATES
550501* - Metabolism- Tracer Techniques
550901 - Pathology- Tracer Techniques
550600 - Medicine