Intracellular location of radionuclide in In-111 tropolone labelled polymorphonuclear leukocytes

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:6782655

Bandyopadhyay, D; Levy, L M; Das, D K

Although autologous PMNs labelled with In-111 tropolone (InT) has been successfully used for the detection of inflammatory lesions in humans, the nature of intracellular binding of this agent has not been properly evaluated. In this present investigation, the distribution of radionuclides in different subcellular fractions was studied and the results were compared to those obtained with cells labelled with In-111 Oxine (InO), the currently used agent of choice. Labelled PMNs were homogenized in STM buffer, pH 7.4 at 4/sup 0/C and subjected to differential centrifugation. Nuclear portion of the cells was purified by sucrose (2.4 M) density centrifugation technic. Purity of the mitochondrial and microsomal fractions were checked by assaying the respective marker enzymes. Purity of the DNA preparations were confirmed by estimating the protein to DNA ratios. All the fractions were counted for radioactivity and the percentage distribution was found. About 30% of nuclear activity was associated with DNA fraction for both preparations. After 20 min incubation of PMNs with InT, all the tropolone was quantitatively recovered in the supernatant indicating that tropolone like oxine is used as a carrier to transport the radionuclide into the cells. The authors conclude much higher (about twice) nuclear binding is observed in case of InT labelled cells as compared to InO preparations. A significant portion of the binding is DNA associated suggesting potential use of the InT labelled PMNs in routine clinical situation.

• Research Organization: Long Island Jewish-Hillside Medical Center, New Hyde Park, NY
• OSTI ID: 6782655
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL NUCLEI
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DNA
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HETEROCYCLIC COMPOUNDS
HUMAN POPULATIONS
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
MATERIALS
MICROSOMES
MINUTES LIVING RADIOISOTOPES
MITOCHONDRIA
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXINE
PATHOLOGICAL CHANGES
PH VALUE
POPULATIONS
PYRIDINES
QUANTITY RATIO
QUINOLINES
RADIOISOTOPES
RADIOPHARMACEUTICALS
SUBCELLULAR DISTRIBUTION
SYMPTOMS
TRACER TECHNIQUES