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/sup 111/Indium-tropolone labeled human PMNs: a rapid method of preparation and evaluation of labeling parameters

Journal Article · · Inflammation (N.Y.); (United States)
OSTI ID:6468223
; ; ; ; ;
Pure polymorphonuclear leukocytes (PMNs) have been isolated from a small amount of human blood by a single-step density gradient centrifugation method using a commercially available Ficoll-Hypaque mixture of density 1.114. The cells were labelled with (/sup 111/In)tropolone in both buffer and plasma. Cell viability, ability to generate superoxide anion, and chemotaxis were found to be unaltered both before and after labeling. The optimum tropolone concentration for labeling was found to be 1 X 10(-4) M. Labeling efficiency was higher at 37 degrees C than at room temperature. Compared to (/sup 111/In)oxine, tropolone preparation both in buffer and plasma resulted in consistently higher yields. Preliminary experiments of in vivo cell viability of the labeled PMNs were carried out in rabbits. The ability of the cells to localize in experimentally produced inflammatory lesions was found to be intact. The method of cell separation and labeling described has been found to be simple and rapid and could easily be incorporated in routine nuclear medicine laboratory practice.
Research Organization:
Long Island Jewish Medical Center, New Hyde Park, NY
OSTI ID:
6468223
Journal Information:
Inflammation (N.Y.); (United States), Journal Name: Inflammation (N.Y.); (United States) Vol. 1; ISSN INFLD
Country of Publication:
United States
Language:
English

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Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHEMICAL PREPARATION
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
ELECTRON CAPTURE RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
LEUKOCYTES
MAMMALS
MAN
MATERIALS
MINUTES LIVING RADIOISOTOPES
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
PATHOLOGICAL CHANGES
PRIMATES
RABBITS
RADIOISOTOPES
SYMPTOMS
SYNTHESIS
USES
VERTEBRATES
VIABILITY