A proposed method for the determination of cerebral regional intermediary glucose metabolism in humans in vivo using specifically labeled /sup 11/C-glucose and positron emission transverse tomography (PETT). I. An animal model with /sup 14/C-glucose and rat brain autoradiography
Based upon data obtained with our arterio-venous technique for the determination of cerebral metabolism in humans in vivo we have proposed a method for the determination of cerebral regional intermediary glucose metabolism in humans in vivo using specifically labeled /sup 11/C-glucose and positron emission transverse tomography (PETT). In it we would give the subject successive intravenous injections of (3,4-/sup 11/C) glucose, (2,5-/sup 11/C) glucose and (1-/sup 11/C) glucose. There would be a 30 min period of continuous PETT measurements following each injection and a 2 hr interval after the first and second injections. The data would be used with suitable equations and algorithms to estimate for each specific region of the subject's brain the dynamics of the Embden-Meyerhof-Parnas (EMP) and the tricarboxylic acid cycle (TCA) metabolic pathways and the incorporation of glucose carbons into lactate, and the extent of dilution of glucose carbons into lactate, and the extent of dilution of glucose carbons in traversing the TCA with their subsequent incorporation into other carbon pools of the brain (ie, glutamate, glutamine, GABA, alanine). Using /sup 14/C as a model for /sup 11/C and autoradiographs made with rat brain slices, we have produced an animal model to demonstrate the feasibility of our proposed method. The resulting autoradiographs have provided evidence of the validity of the predictions made from our arterio-venous data. The model was employed to show the selective reductions in the rates of incorporation of specific carbon atoms of glucose into regions of the rat brain and evidence of altered metabolic pathways following a single electroconvulsive shock (ECS) and after a series of nine ECS.
- Research Organization:
- New York State Rockland Research Institute, Orangeburg
- OSTI ID:
- 6780707
- Journal Information:
- J. Neurosci. Res.; (United States), Vol. 7:1
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
CARBON 11
DIAGNOSTIC USES
CEREBRUM
PHYSIOLOGY
GLUCOSE
METABOLISM
BIOLOGICAL PATHWAYS
CARBON 14 COMPOUNDS
IN VIVO
LABORATORY ANIMALS
PATIENTS
POSITRON COMPUTED TOMOGRAPHY
ALDEHYDES
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
BRAIN
CARBOHYDRATES
CARBON ISOTOPES
CENTRAL NERVOUS SYSTEM
COMPUTERIZED TOMOGRAPHY
DIAGNOSTIC TECHNIQUES
EMISSION COMPUTED TOMOGRAPHY
EVEN-ODD NUCLEI
HEXOSES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MINUTES LIVING RADIOISOTOPES
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
SACCHARIDES
TOMOGRAPHY
USES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques