Characterization of the interaction between local cerebral metabolic rate for glucose and acid-base index in ischemic rat brain employing a double-isotope methodology

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5227316

Peek, K E.H.

The association between increases in cerebral glucose metabolism and the development of acidosis is largely inferential, based on reports linking hyperglycemia with poor neurological outcome, lactate accumulation, and the severity of acidosis. We measured local cerebral metabolic rate for glucose (lCMRglc) and an index of brain pH-the acid-base index (ABI)-concurrently and characterized their interaction in a model of focal cerebral ischemia in rats in a double-label autoradiographic study, using ({sup 14}C)2-deoxyglucose and ({sup 14}C)dimethyloxazolidinedione. Computer-assisted digitization and analysis permitted the simultaneous quantification of the two variables on a pixel-by-pixel basis in the same brain slices.

• Research Organization: Texas Univ., Houston, TX (USA). Health Science Center
• OSTI ID: 5227316
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
METABOLISM
ISCHEMIA
PATHOGENESIS
AUTORADIOGRAPHY
CARBON 14 COMPOUNDS
CEREBRAL CORTEX
CHEMICAL COMPOSITION
COMPUTER CALCULATIONS
DOUBLE LABELLING
HYPERGLYCEMIA
PH VALUE
RATS
ALDEHYDES
ANIMALS
BODY
BRAIN
CARBOHYDRATES
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CEREBRUM
DISEASES
HEXOSES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RODENTS
SACCHARIDES
VASCULAR DISEASES
VERTEBRATES
550501* - Metabolism- Tracer Techniques
550901 - Pathology- Tracer Techniques