Intracochlear microprobe analysis

Journal Article · Thu Apr 01 04:00:00 EST 1982 · Laryngoscope; (United States)

DOI:https://doi.org/10.1288/00005537-198204000-00006· OSTI ID:6778862

Bone, R C; Ryan, A F

Energy dispersive x-ray analysis (EDXA) or microprobe analysis provides cochlear physiologists with a means of accurately assessing relative ionic concentrations in selected portions of the auditory mechanism. Rapid freezing followed by lyophilization allows the recovery of fluid samples in crystalline form not only from perilymphatic and endolymphatic spaces, but also from much smaller subregions of the cochlea. Because samples are examined in a solid state, there is no risk of diffusion into surrounding or juxtaposed fluids. Samples of cochlear tissues may also be evaluated without the danger of intercellular ionic diffusion. During direct visualization by scanning electron microscopy, determination of the biochemical makeup of the material being examined can be simultaneously, assuring the source of the data collected. Other potential advantages and disadvantages of EDXA are reviewed. Initial findings as they relate to endolymph, perilymph, stria vascularis, and the undersurface of the tectorial membrane are presented.

• Research Organization: Auditory Physiology Laboratory, Veterans Administration Hospital, La Jolla, CA
• OSTI ID: 6778862
• Journal Information: Laryngoscope; (United States), Journal Name: Laryngoscope; (United States) Vol. 92:4; ISSN LARYA
• Country of Publication: United States
• Language: English

Related Subjects

550602 -- Medicine-- External Radiation in Diagnostics-- (1980-)
551000* -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AUDITORY ORGANS
BIOCHEMISTRY
BODY
CHEMICAL ANALYSIS
CHEMISTRY
ELECTRON MICROPROBE ANALYSIS
ELECTRON MICROSCOPY
IONIC COMPOSITION
MICROANALYSIS
MICROSCOPY
NONDESTRUCTIVE ANALYSIS
ORGANS
PHYSIOLOGY
SCANNING ELECTRON MICROSCOPY
SENSE ORGANS
X-RAY EMISSION ANALYSIS