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Defective natural killer (NK) cell activity in a rat burn model

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6778157
In this report they present data on the spontaneous cytotoxic (NK) activity of scalded rat splenic lymphocytes. Sprague-Dawley rats (220-250 grams) were given 40% total body surface area full thickness burn injury by immersing the anesthetized rats in 90/sup 0/C water. Groups of rats were sacrificed at 1,2,4 and 7 day post burn (DPB). Splenic lymphocytes were tested for their ability to lyse YAC-1 target cells in a /sup 51/Cr release assay by incubating /sup 51/Cr-labelled YAC cells with lymphocytes at effector to target (E:T) ratios of 200:1 to 12:1. NK activity of the splenic lymphocytes from scalded rats was observed to be suppressed at all E:T ratios as early as 1 DPB. This decrease was observed at all time periods of study. Their present observations and previous findings of suppressed lymphoproliferative responses in scalded rats support similar findings in human studies, thereby making the rat a good model of immune function studies following thermal injury.
Research Organization:
Shriners Burns Institute, Galveston, TX
OSTI ID:
6778157
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
Country of Publication:
United States
Language:
English