Enhanced lysis of herpes simplex virus type 1-infected mouse cell lines by NC and NK effectors

Journal Article · Thu May 01 04:00:00 EDT 1986 · J. Immunol.; (United States)

OSTI ID:6936195

Colmenares, C; Lopez, C

Spontaneously cytotoxic murine lymphocytes lysed certain cell types infected by herpes simplex virus type 1 (HSV-1) better than uninfected cells. Although HSV-1 adsorbed to the surface of all the target cells, those in which the virus replicated more efficiently were lysed to a greater extent. As targets, the authors used cell lines that, when uninfected, were spontaneously lysed by NK cells (YAC-1) or by NC cells (WEHI-164). They also used a fibroblastoid cell line (M50) and a monocytic tumor line (PU51R), which were not spontaneously killed. NK cells lysed HSV-1-infected YAC cells better than uninfected cells, and an NC-like activity selectively lysed HSV-1-infected WEHI cells. These findings were consistent with the results of experiments performed to define the role of interferon in induction of virus-augmented cytolysis. Increased lysis of YAC-HSV and PU51R-HSV was entirely due to interferon activation and was completely abolished by performing the /sup 51/Cr-release assay in the presence of anti-interferon serum. The data show that HSV-1 infection of NK/NC targets induces increased cytotoxity, but the effector cell responsible for lysis is determined by the uninfected target, or by an interaction between the virus and target cell, rather than by a viral determinant alone.

• Research Organization: Sloan-Kettering Institute for Cancer Research, New York, NY
• OSTI ID: 6936195
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:9; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL CULTURES
CELL KILLING
CELL WALL
CHEMICAL ANALYSIS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FIBROBLASTS
GROWTH FACTORS
HERPES SIMPLEX
INFECTIOUS DISEASES
INTERFERON
INTERMEDIATE MASS NUCLEI
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
LYSIS
MAMMALS
MATERIALS
MICE
MICROORGANISMS
MITOGENS
NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIO-RELEASE ANALYSIS
RADIOISOTOPES
RODENTS
SKIN DISEASES
SOMATIC CELLS
TUMOR CELLS
VERTEBRATES
VIRAL DISEASES
VIRUSES