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Title: Natural killer (NK) activity of pit cells perfused from livers of rats treated with ethanol

Abstract

The liver is the major site of ethanol (ETOH) metabolism. Liver sinusoids contain lymphocytes with NK activity. The authors treated LEW rats for 2 weeks with i.p. injection of 1.25 ml 25% ETOH/kg 3 times/week and 5% ETOH in drinking water. Livers were perfused at 5-fold physiological pressure and cells obtained were banded on 1.077 density Ficoll. Their cytotoxicity was tested against {sup 51}Cr-labeled YAC-1 or U937 and compared to spleen and blood lymphocytes. In untreated rats, pit cell NK activity was 2-fold that of splenic lymphocytes and 4-fold that of blood lymphocytes. Compared to controls, ETOH-treated rats exhibited a 30 to 90% rise in pit cell NK activity detected with YAC-1 or U937 targets. The pit cell enhanced NK activity in ETOH-treated rats was further increased if polyinosinicpolycytidilic acid was injection i.p. 18 hours before the assay. Blood and spleen lymphocyte NK activity of ETOH-treated rats was also greater than in controls. There was no evidence that ETOH merely redistributed lymphocytes among the tissues. Although ETOH acutely inhibits NK activity in vitro, chronic ETOH increases in vivo.

Authors:
; ; ;  [1]
  1. (National Inst. of Alcohol Abuse and Alcoholism, Rockville, MD (United States) Univ. of Arkansas Medical Sciences, Little Rock (United States))
Publication Date:
OSTI Identifier:
5905513
Alternate Identifier(s):
OSTI ID: 5905513
Report Number(s):
CONF-9104107--
Journal ID: ISSN 0892-6638; CODEN: FAJOE
Resource Type:
Conference
Resource Relation:
Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States); Journal Volume: 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ETHANOL; BIOLOGICAL EFFECTS; LYMPHOCYTES; CELL PROLIFERATION; BLOOD; CHROMIUM 51; LIVER; METABOLISM; PERFUSED ORGANS; RATS; SPLEEN CELLS; TRACER TECHNIQUES; ALCOHOLS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY; BODY FLUIDS; CHROMIUM ISOTOPES; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; GLANDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; NUCLEI; ORGANIC COMPOUNDS; ORGANS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology

Citation Formats

Albornoz, L., Jones, J.M., Crutchfield, C., and Veech, R.L.. Natural killer (NK) activity of pit cells perfused from livers of rats treated with ethanol. United States: N. p., 1991. Web.
Albornoz, L., Jones, J.M., Crutchfield, C., & Veech, R.L.. Natural killer (NK) activity of pit cells perfused from livers of rats treated with ethanol. United States.
Albornoz, L., Jones, J.M., Crutchfield, C., and Veech, R.L.. Mon . "Natural killer (NK) activity of pit cells perfused from livers of rats treated with ethanol". United States. doi:.
@article{osti_5905513,
title = {Natural killer (NK) activity of pit cells perfused from livers of rats treated with ethanol},
author = {Albornoz, L. and Jones, J.M. and Crutchfield, C. and Veech, R.L.},
abstractNote = {The liver is the major site of ethanol (ETOH) metabolism. Liver sinusoids contain lymphocytes with NK activity. The authors treated LEW rats for 2 weeks with i.p. injection of 1.25 ml 25% ETOH/kg 3 times/week and 5% ETOH in drinking water. Livers were perfused at 5-fold physiological pressure and cells obtained were banded on 1.077 density Ficoll. Their cytotoxicity was tested against {sup 51}Cr-labeled YAC-1 or U937 and compared to spleen and blood lymphocytes. In untreated rats, pit cell NK activity was 2-fold that of splenic lymphocytes and 4-fold that of blood lymphocytes. Compared to controls, ETOH-treated rats exhibited a 30 to 90% rise in pit cell NK activity detected with YAC-1 or U937 targets. The pit cell enhanced NK activity in ETOH-treated rats was further increased if polyinosinicpolycytidilic acid was injection i.p. 18 hours before the assay. Blood and spleen lymphocyte NK activity of ETOH-treated rats was also greater than in controls. There was no evidence that ETOH merely redistributed lymphocytes among the tissues. Although ETOH acutely inhibits NK activity in vitro, chronic ETOH increases in vivo.},
doi = {},
journal = {FASEB Journal (Federation of American Societies for Experimental Biology); (United States)},
number = ,
volume = 5:4,
place = {United States},
year = {Mon Mar 11 00:00:00 EST 1991},
month = {Mon Mar 11 00:00:00 EST 1991}
}

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  • The authors examined NK and OKT3 augmented NK activity in 14 IDDM patients and 15 age matched controls (28 +/- 6 vs 28.5 +/- 6 yrs respectively) to evaluate this aspect of antigen nonspecific immunity. NK and augmented NK function were examined at various target to effector cell ratios (E/T) using the K562 cell line (as target) in a 4 hr /sup 51/Cr release assay. Islet cell antibodies (ICA) were determined by standard methods. All of the diabetics (mean duration of disease 16 yrs) and controls were ICA (-). Their observations indicate that there is no significant difference between diabeticmore » and control subjects in NK activity. The ability of OKT3 to augment NK activity (by a T-cell mediated process) is also not significantly different between the two groups. An abnormal immune response to beta cells has a central role in the pathogenesis of IDDM. The nature of this autoimmune defect is unclear. The authors' present observations indicate that antigen nonspecific immune function may be normal in patients with IDDM. They propose that IDDM is a disease only of abnormal antigen specific immunoregulation.« less
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  • Mice that have been injected with /sup 89/Sr have fairly normal B and T cell function, but are abnormal in that they lack natural killer (NK) activity and other functions that require an intact bone marrow. These mice also have an increased potential for suppressor cell activity. We had previously shown that spleen cells from /sup 89/Sr-treated mice could transfer low NK activity and increased suppressor cell function to lethally irradiated syngeneic recipients. To investigate the mechanisms involved in perpetuating these defects, groups of normal spleen or bone marrow cells. Recipients were assayed for their NK activity and suppressor cellmore » function 5 to 14 wk later. it was found that the addition of normal cells in the donor inoculum resulted in normal NK activity. This indicates that low NK activity in /sup 89/Sr-treated mice was not due to the presence of a suppressor cell that prevented NK cell generation. It was additionally found that low NK activity in recipient mice could be boosted by interferon inducers. This would indicate that NK activity in the recipients was not due to a lack of interferon-sensitive pre-NK cells. Suppressor cell function in recipient mice depended on the type and number of normal cells in the donor inoculum. Bone marrow cells were very efficient in overcoming the tendency to produce suppressor cells. It took approximately 20 times more normal spleen cells to produce the same results. The implications of these findings are discussed.« less
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