Different migratory patterns of blood, splenic, thymic lymphocytes, T and NK cells labeled with In-111 tropolone in rats

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:6613728

Gagne, G; Garcia, A M; McAfee, J G

Labeled cells harvested from different lymphoid organs have been used to study lymphocyte migration in experimental animals, but the influence of the source organ on their biodistribution is largely unknown. The authors harvested peripheral blood cells (88% lymphocytes), splenic cells, splenic T cells after elimination of BETA cells by nylon wool column, thymic cells and natural killer (NK) enriched cells. For the last preparation, spleen cells were obtained from neonatal thymectomized rats, nylon wool column filtered, and centrifuged on Percoll, to obtain 27% NK cells. These 5 different cell preparations were labeled with (In-111 tropolone (<10..mu..Ci/10/sup 8/ cells) and their blood clearance and localization were compared in Wistar rats. Peripheral blood cells maintained a high blood level up to 24 hr. (27%), similar to previous studies in humans. They reached a high concentration in lymph nodes (25%), with comparatively low levels in the spleen and liver. In contrast, cells of splenic or thymic origin disappeared rapidly from the blood and sequestered temporarily in the lungs. Thymic cells were sequestered in the liver, and concentrated poorly in lymph nodes. Splenic T cells had a relatively high concentration in lymph nodes and marrow. NK enriched cells maintained a relatively high level in the blood, a low concentration in lymph nodes, and sequestered chiefly in the liver. These results indicate that the source organ from which lymphocytes are extracted is a major determinant in their localization.

• Research Organization: Upstate Medical Center, Syracuse, NY
• OSTI ID: 6613728
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HEMATOPOIETIC SYSTEM
IMMUNOLOGY
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LEUKOCYTES
LIVER
LUNGS
LYMPH VESSELS
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MEDICINE
MIGRATION
MINUTES LIVING RADIOISOTOPES
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOIMMUNOLOGY
RADIOIMMUNOTHERAPY
RADIOISOTOPES
RADIOLOGY
RADIONUCLIDE KINETICS
RADIOPHARMACEUTICALS
RADIOTHERAPY
RATS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SPLEEN
THERAPY
TISSUE DISTRIBUTION
TISSUES
VERTEBRATES