Reversion at the his1 locus of yeast. [X radiation]

Journal Article · Wed Nov 01 04:00:00 EST 1978 · Genetics; (United States)

OSTI ID:6776577

Fogel, S; Lax, C; Hurst, D D

The his1 gene (chromosome V) of Saccharomyces cerevisiae specifies phosphoribosyl transferase (E.C.2.4.2.17), the first enzyme of histidine biosynthesis. This hexameric enzyme has both catalytic and regulatory functions. The spontaneous reversion rates of seven his1 mutations were studied. The reversion rates of the alleles at the proximal end of the locus (relative to the centromere) were about 50-fold higher than distal alleles. Spontaneous reversion to prototrophy was studied in diploids homoallelic for each of the seven his1 mutations. Based on tetrad analysis, the prototrophy revertants could be assigned to three classes: revertant tetrads that carried a prototrophic allele indistinguishable from wild type; revertant tetrads that carried a prototrophic allele characterized by histidine excretion and feedback resistance; and revertant tetrads that did not contain a prototrophic spore, but rather a newly derived allele that complemented the original allele intragenically. Four of the seven his1 mutations produced the excretor revertant class, and two mutations produced the complementer revertant class. The significance of these findings to our understanding of gene organization and the catalytic and regulatory functions of gene products are discussed.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 6776577
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 90:3; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
FUNGI
GENES
IONIZING RADIATIONS
MICROORGANISMS
MUTATIONS
PLANTS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
X RADIATION
YEASTS