Interacting genes that affect microtubule function in Drosophila melanogaster: Two classes of mutation revert the failure to complement between hay sup nc2 and mutations in tubulin genes
- Univ. of Colorado, Boulder (USA)
The recessive male sterile mutation hay{sup nc2} of Drosophila melanogaster fails to complement certain {beta}{sub 2}-tubulin and {alpha}-tubulin mutations, suggesting that the haywire product plays a role in microtubule function, perhaps as a structural component of microtubules. The genetic interaction appears to require the presence of the aberrant product encoded by hay{sup nc2}, which may act as a structural poison. Based on this observation, the authors have isolated ten new mutations with EMS that revert the failure to complement between hay{sup nc2} and B2t{sup n}. The revertants tested behaved as intragenic mutations of hay in recombination tests, and feel into two phenotypic classes, suggesting two functional domains of the hay gene product. Some revertants were hemizygous viable and less severe than hay{sup nc2} in their recessive phenotype. These mutations might revert the poison by restoring the aberrant product encoded by the hay{sup nc2} allele to more wild-type function. Most of the revertants were recessive lethal mutations, indicating that the hay gene product is essential for viability. These more extreme mutations could revert the poison by destroying the ability of the aberrant haywire{sup nc2} product to interact structurally with microtubules. Flies heterozygous for the original hay{sup nc2} allele and an extreme revertant show defects in both the structure and the function of the male meiotic spindle.
- OSTI ID:
- 6521470
- Journal Information:
- Genetics; (USA), Vol. 125:1; ISSN 0016-6731
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
EMS
GENETIC EFFECTS
MICROTUBULES
MOLECULAR STRUCTURE
DROSOPHILA
GENE RECOMBINATION
LETHAL MUTATIONS
PHENOTYPE
PROTEINS
RECESSIVE MUTATIONS
REVERTANTS
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
DIPTERA
ESTERS
FLIES
FRUIT FLIES
INSECTS
INVERTEBRATES
MUTAGENS
MUTANTS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
SULFONIC ACID ESTERS
560300* - Chemicals Metabolism & Toxicology