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Herpes simplex virus-mediated human hypoxanthine-guanine phosphoribosyltransferase gene transfer into neuronal cells

Journal Article · · Mol. Cell. Biol.; (United States)
DOI:https://doi.org/10.1128/MCB.8.1.457· OSTI ID:6772598
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The virtually complete deficiency of the purine salvage enzyme hypoxanthine-guanine phosphoribosyltransferase (HPRT) results in a devastating neurological disease, Lesch-Nyhan syndrome. Transfer of the HPRT gene into fibroblasts and lymphoblasts in vitro and into hematopoietic cells in vivo has been accomplished by other groups with retroviral-derived vectors. It appears to be necessary, however, to transfer the HPRT gene into neuronal cells to correct the neurological dysfunction of this disorder. The neurotropic virus herpes simplex virus type 1 has features that make it suitable for use as a vector to transfer the HPRT gene into neuronal tissue. This report describes the isolation of an HPRT-deficient rat neuroma cell line, designated B103-4C, and the construction of a recombinant herpes simplex virus type 1 that contained human HPRT cDNA. These recombinant viruses were used to infect B103-4C cells. Infected cells expressed HPRT activity which was human in origin.
Research Organization:
Departments of Internal Medicine, Univ. of Michigan Medical School, Ann Arbor, MI (US)
OSTI ID:
6772598
Journal Information:
Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 8:1; ISSN MCEBD
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
ENZYME ACTIVITY
ENZYMES
FIBROBLASTS
GENES
GENETIC ENGINEERING
GLYCOSYL TRANSFERASES
HERPES SIMPLEX
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
IN VITRO
INFECTIOUS DISEASES
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEDICINE
NERVE CELLS
NEUROLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PENTOSYL TRANSFERASES
PHENOTYPE
RECOMBINANT DNA
SKIN DISEASES
SOMATIC CELLS
TECHNOLOGY ASSESSMENT
TRANSFERASES
VIRAL DISEASES