Radioimmunoassay of corticotropin from plasma

Journal Article · Mon Nov 01 04:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6766617

Gutkowska, J; Julesz, J; St-Louis, J; Genest, J

We describe a specific and highly sensitive radioimmunoassay for corticotropin (ACTH) in human plasma. As little as 1.9 pg of circulating ACTH can be detected per milliliter (0.44 pmol/L). ACTH is first extracted from plasma samples by adsorption onto pretreated Vycor glass powder, eluted from the adsorbent by acetone, and then lyophilized. For purification of /sup 125/I-labeled ACTH, a small octadecasilyl silica column is used. The mean (and SD) concentration of ACTH in plasma from 18 ostensibly normal subjects was 10.3 (4.3) pmol/L. After overnight suppression with dexamethasone in seven of these normal subjects, their ACTH values fell to 2.62 (1.3) pmol/L (p less than 0.0005). This assay will permit clinical evaluation of ACTH plasma concentrations in clinical investigation and of the effects of drug administration on circulating ACTH.

• Research Organization: Clinical Research Institute of Montreal, Quebec, Canada
• OSTI ID: 6766617
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 28:11; ISSN CLCHA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACTH
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CHEMISTRY
BLOOD PLASMA
BODY FLUIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
NUCLEI
ODD-EVEN NUCLEI
PATIENTS
PEPTIDE HORMONES
PHYSIOLOGY
PITUITARY HORMONES
RADIOIMMUNOLOGY
RADIOISOTOPES