Radioimmunoassay for plasma corticotropin in frogs (Rana esculenta L.)

Journal Article · Sat Mar 01 04:00:00 EDT 1975 · Gen. Comp. Endocrinol., v. 25, no. 3, pp. 313-322

DOI:https://doi.org/10.1016/0016-6480(75)90121-5· OSTI ID:4041475

Vaudry, H; Vague, P; Dupont, W; Leboulenger, F; Vaillant, R

A radioimmunoassay technique has been developed for measuring frog plasma corticotropin (ACTH) without prior extraction. Using synthetic porcine ACTH as a reference standard, $sup 131$I-labeled synthetic human ACTH (sp act greater than 500 mCi/mg) as tracer and rabbit anti-porcine ACTH serum, the lower measurable value was estimated at about 4 pg ACTH. Only human and porcine ACTH, ACTH, and frog pituitary ACTH reacted with the rabbit anti-porcine ACTH serum. No cross-reactivity has been found with synthetic ACTH, $alpha$MSH, and bovine $beta$MSH. Appearance of damaged $sup 131$I-h ACTH components after storage in plasma solutions was followed for 7 days. The conditions making it possible to reduce ACTH damage have been ascertained. The average plasma corticotropin level (+- CI) was found to be 38.8 +- 7.8 pg/ml without any significant difference between males and females. These results suggest that frog ACTH secretion has much in common with mammalian secretions. (auth)

• Research Organization: Laboratoire d'Endocrinologie, Mont-Saint-Aignan, France
• Sponsoring Organization: USDOE
• NSA Number: NSA-33-029627
• OSTI ID: 4041475
• Journal Information: Gen. Comp. Endocrinol., v. 25, no. 3, pp. 313-322, Journal Name: Gen. Comp. Endocrinol., v. 25, no. 3, pp. 313-322; ISSN GCENA
• Country of Publication: United States
• Language: English

Related Subjects

*ACTH-- RADIOIMMUNOASSAY
560151* --Biomedical Sciences
Applied Studies--Radiation Effects--Radiation Effects on Animals--Man
BLOOD PLASMA
FROGS
IMMUNE SERUMS
IODINE 131
LABELLED COMPOUNDS
N48510* --Life Sciences--Radiation Effects on Animals--Man