Heterologous radioimmunoassay for arginine vasopressin

Journal Article · Sun Feb 01 04:00:00 EST 1976 · J. Lab. Clin. Med., v. 87, no. 2, pp. 338-344

OSTI ID:4030799

Shimamoto, K; Murase, T; Yamaji, T

A sensitive and specific radioimmunoassay for arginine vasopressin (AVP) was developed utilizing the antisera against lysine vasopressin (LVP) in combination with a labeled AVP. The assay employs an acetone extraction procedure and detects as little as 0.8 pg. per millimeter of AVP in human plasma. In normal subjects, the mean (+- S.D.) plasma concentration of AVP was 4.9 +- 1.2 pg. per milliliter after fluid deprivation and 1.2 +- 0.4 pg. per milliliter after water loading. Plasma AVP levels correlated significantly with plasma osmolalities. In four patients with diabetes insipidus, plasma AVP concentrations ranged from less than 0.8 to 1.2 pg. per milliliter, whereas six patients with the syndrome of inappropriate ADH secretion showed plasma levels of AVP which correspond to those of the dehydrated state in normal subjects or greater, although plasma osmolalities were low in all cases. It was concluded that the present radioimmunoassay method for AVP provides a useful way of assessing neurohypophyseal function in man. (auth)

• Research Organization: Tokyo Univ.
• NSA Number: NSA-33-026357
• OSTI ID: 4030799
• Journal Information: J. Lab. Clin. Med., v. 87, no. 2, pp. 338-344, Journal Name: J. Lab. Clin. Med., v. 87, no. 2, pp. 338-344; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

*VASOPRESSIN-- RADIOIMMUNOASSAY
550601* --Biomedical Sciences
Basic Studies--Medicine-- Unsealed Radionuclides in Diagnostics
ARGININE
BLOOD PLASMA
IODINE 125
LYSINE
N48830* --Life Sciences--New Tracer Techniques--Other
PATIENTS
TRACER TECHNIQUES