Radioimmunoassay of Arginine Vasopressin in Human Plasma

Journal Article · Mon Oct 01 04:00:00 EDT 1973 · Journal of Clinical Endocrinology and Metabolism

DOI:https://doi.org/10.1210/jcem-37-4-616· OSTI ID:4419050

HUSAIN, M. KAZIM; FERNANDO, NIHAL; SHAPIRO, MENACHEM; KAGAN, AVIR; GLICK, SEYMOUR M.

A highly sensitive specific radioimmunoassay (RIA) method for the measurement of arginine vasopressin (AVP) is described. The sensitivity of the assay is 0.1 pg/ml. A simple extraction technique from plasma is described with- a consistent recovery of about 65 to 70%, and without alteration in the immunoreactivity of the extracted hormone. AVP levels, uncorrected for recovery, ranged between 1.3 and 8.5 pg/ml (mean 3.7 pg/ml) in humans after 12 to 25 hr of water deprivation, and were consistently less than 1.2 pg/ml after water loading. AVP levels were undetectable in 6 patients with diabetes insipidus. Smoking was followed by up to several hundred-fold increases in plasma AVP. Plasma AVP levels in animals also rose with dehydration, increased plasma hypertonicity and hypovolemia and fell with hydration. (auth)

• Research Organization: Coney Island Hospital, Brooklyn
• Sponsoring Organization: USDOE
• NSA Number: NSA-29-000875
• OSTI ID: 4419050
• Journal Information: Journal of Clinical Endocrinology and Metabolism, Journal Name: Journal of Clinical Endocrinology and Metabolism Journal Issue: 4 Vol. 37; ISSN 0021-972X
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*VASOPRESSIN-- RADIOIMMUNOASSAY
ARGININE
BLOOD PLASMA
IODINE 125
N48830* --Life Sciences--New Tracer Techniques--Other
TRACER TECHNIQUES