Comparison of radiolabeled monoclonal antibodies in an in vitro tissue binding assay

Conference · Wed May 01 04:00:00 EDT 1985 · J. Nucl. Med.; (United States)

OSTI ID:6762072

Rusckowski, M; Virzi, F; Hnatowich, D J

The authors previously described a simple in vitro assay for evaluating radiolabeled monoclonal antibodies (Abs) as an aid in selecting for clinical trials those with superior tissue binding properties. Tumor or normal tissues as 1 mm cubes are incubated in microtitre-plate wells containing media and indium-111 labeled Abs and, following rinsing, are counted to measure binding. The assay has now been used to evaluate 4 anti-colorectal tumor Abs against 6 human colorectal tumor tissues and normal liver and colon with an additional Ab (anti-PAP) and tissue (CX-1, an established colorectal tumor) as controls. In addition, 4 anti-breast tumor Abs were evaluated against 7 human tumor tissues and normal tissues from 8 organs. Differences in binding of one Ab to different tissues and of different Abs to one tissue were observed in both studies which were usually for larger than the range of repeat measurements. Lower binding was usually observed for the control Ab and for normal tissues. However, increased binding of anti-tumor Abs occasionally occurred in normal tissues especially in spleen and pancreas tissue with anti-breast tumor Abs and in normal colon tissue with one anti-colorectal tumor Ab. A comparison with immunoperoxidase staining in the same tissues with the anti-breast tumor Abs showed a pattern of specificity not dissimilar from that shown by the assay.

• Research Organization: Univ. of Massachusetts Medical Center, Worcester, MA
• OSTI ID: 6762072
• Report Number(s): CONF-850611-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 26:5
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
BIOLOGICAL MARKERS
BODY
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVALUATION
FUNCTIONS
GASTROINTESTINAL TRACT
GLANDS
IMMUNOLOGY
IN VITRO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
INTESTINES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LARGE INTESTINE
LIVER
MAMMARY GLANDS
MEDICINE
METABOLISM
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOIMMUNOLOGY
RADIOIMMUNOTHERAPY
RADIOISOTOPES
RADIOLOGY
RADIOPHARMACEUTICALS
RADIOTHERAPY
REACTION KINETICS
RETENTION FUNCTIONS
THERAPY
UPTAKE