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Characterization and immunotherapeutic potential of a monoclonal antibody against a ras oncogene transformed cell line

Thesis/Dissertation ·
OSTI ID:6701131

Transformed cells express cell surface antigens not present, or present in diminished amounts on normal cells. Monoclonal antibodies can be used to identify and biochemically characterize tumor-associated antigens. Monoclonal antibody (MoAb) 45-2D9 was produced by immunization of BALB/c mice with a transformed cell line (45-2D9) induced by transfection of NIH 3T3 cells with a c-H-ras oncogene in DNA isolated from a human lung carcinoma. By immunoperoxidase staining, this antibody binds to the 45-342 cells as well as to the ras transformed primary and 3 secondary transfectants, including the one used to induce 45-342, but not to other ras transformed cell lines. Murine tumors as well as human fetal and most normal adult tissues are not stained. This antibody does bind to a variety of human tumors, including lung adenocarcinomas, as well as breast, colon and esophageal carcinomas. The ability of MoAb 45-2D9 to target ricin toxin A chain (RTA) and radio-isotopes to gp74 expressing cells was investigated. An immunotoxin generated by conjugating RTA to MoAb 45-2D9 inhibits protein and DNA synthesis by the 45-342 cells. Radiolabeled antibody specifically localizes to and can be used to image subcutaneous and pulmonary gp74 expressing tumors in nu/nu mice. Monoclonal antibodies against oncogene transformed cell lines may be useful for the detection and characterization of tumor-associated antigens as well as for the development of new tumor therapeutic and diagnostic reagents.

Research Organization:
George Washington Univ., Washington, DC (USA)
OSTI ID:
6701131
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BODY
CARCINOMAS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC USES
DIGESTIVE SYSTEM
DISEASES
DNA REPLICATION
ELECTRON CAPTURE RADIOISOTOPES
ESOPHAGUS
FIBROBLASTS
GENES
GLANDS
IMMUNOTHERAPY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LUNGS
MAMMALS
MAMMARY GLANDS
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
NUCLEIC ACID REPLICATION
ODD-EVEN NUCLEI
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
THERAPY
TUMOR CELLS
USES
VERTEBRATES