Cell-surface associated with transformation of human hepatocytes to the malignant phenotype

Journal Article · Sun May 01 00:00:00 EDT 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.9.3140· OSTI ID:6207472

Wilson, B; Ozturk, M; Takahashi, H; Motte, P; Kew, M; Isselbacher, K J; Wands, J R

Hepatocellular carcinoma is one of the leading causes of cancer death in the world. To understand the cellular changes associated with transformation of hepatocytes to the malignant state, the authors have made several libraries of monoclonal antibodies against the hepatocellular carcinoma cell line FOCUS and have found six antibodies (AF-20, SF-25, SF-31, SF-90, XF-4, and XF-8) that recognize antigens expressed at consistently higher levels on hepatoma cells. They have studied malignant and nontransformed liver tissue from the same individual by using direct /sup 125/I-labeled antibody binding and immunoperoxidase staining techniques. For each of these antibodies, they found striking increases in antigen expression on the transformed tissues. These antigens were found to be expressed throughout the tumor and on distant metastases, with little, if any, expression on the nontransformed adjacent liver. These antibodies demonstrate that hepatic transformation may be accompanied by stereotyped and predictable antigenic changes. The uniformity of such antigenic changes suggests an association between these cell-surface alterations and the malignant transformation process.

• Research Organization: Harvard Medical School, Boston, MA (USA)
• OSTI ID: 6207472
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:9; ISSN PNASA
• Country of Publication: United States
• Language: English

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550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
AMINES
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGY
BODY
CARBOHYDRATES
CARCINOMAS
CELL CONSTITUENTS
CELL MEMBRANES
CELL TRANSFORMATIONS
CYTOLOGY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
GLUCOSAMINE
HALIDES
HALOGEN COMPOUNDS
HEXOSAMINES
HEXOSES
IMMUNOASSAY
IMMUNOLOGY
INORGANIC PHOSPHORS
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIVER
LIVER CELLS
MEMBRANES
MONOCLONAL ANTIBODIES
MONOSACCHARIDES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SACCHARIDES
SODIUM COMPOUNDS
SODIUM IODIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS