Nucleotide sequence of messenger RNA encoding V[sub H]DJ[sub H] and V[sub K]J[sub K] of a highly conserved idiotype-defined primary response anti-hapten antibody

Journal Article · Tue Dec 15 04:00:00 EST 1992 · Journal of Immunology; (United States)

OSTI ID:6762013

Lou, S C; Winter, D; Mayers, G L; Bankert, R B ^[1]

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1. Roswell Park Cancer Inst., Buffalo, NY (United States)

The primary humoral immune response of mice to the hapten phthalate (Xmp) is focused upon two adjacent immunodominant negatively charged carboxyl groups on a benzene ring that are in positions meta and para to the azolinkage (i.e., Xmp) to the protein carrier keyhole limpet hemocyanin. A significant fraction of the anti-Xmp antibodies raised in several different inbred mouse strains (BALB/c, DBA/2, A/HeHa; C3H, and SM/J), and many wild mouse populations express a cross-reactive Id, CRI[sub Xmp-1]. This CRI[sub Xmp-1] is conspicuously absent in C57BL/6 mice. In order to obtain a better understanding of the events and parameters that influence the selection and regulation of the primary response B cell repertoire, and to explore the structural basis of Ag binding, the authors have determined the nucleotide sequence of the entire V region gene complexes, which encode the H and L chains of these highly conserved and dominant CRI[sub Xmp-1+] antibodies. Their data establish that the H chain gene complex consists of a single V[sub H] germ-line gene that is identical to V[sub H] Oxazolone-1, encoding the H chain of another highly conserved and dominant cross-reactive Id family associated with the primary response to Oxazolone. The amino acid sequence predicted from the nucleotide sequence of the V[sub K]J[sub K] from Xmp-specific CRI[sub Xmp-1+] hybridomas is identical to the sequence of the anti-arsonate antibody 1210.7, which is the prototype of another Id family (CRI) that is conserved and dominant in BALB/c mice. 60 refs., 3 figs., 3 tabs.

• OSTI ID: 6762013
• Journal Information: Journal of Immunology; (United States), Journal Name: Journal of Immunology; (United States) Vol. 149:12; ISSN 0022-1767; ISSN JOIMA3
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DNA SEQUENCING
GENE REGULATION
GENES
IMMUNE REACTIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEOTIDES
ORGANIC COMPOUNDS
PROTEIN STRUCTURE
PROTEINS
RECEPTORS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS