Allelic differences in the V{sub H}Ox-1 gene explain the absence of a B cell clonal dominance in the primary response of C57BL/6 mice to phthalate
Journal Article
·
· Journal of Immunology
OSTI ID:160163
- Roswell Park Cancer Institute, Buffalo, NY (United States); and others
A highly conserved Id (CRI{sub Xmp-1}) associated with the murine (BALB/c) humoral immune response to the hapten phthalate (Xmp) is conspicuously absent in C57BL/6 mice. The absence of this Id in C57BL/6 mice is shown here to be due to the absence of the appropriate V{sub H} gene (V{sub H}Ox-1) usage in the Xmp response. To determine whether the failure to utilize this V{sub H} was due to an active suppression or to the lack of the requisite V{sub H} gene in the available repertoire, V{sub H}Ox-1 gene-specific primers were used to amplify the germ-line V{sub H}Ox-1 gene from genomic DNA from BALB/c and C57BL/6 mice. The germ-line coding sequence of the C57BL/6 allele of the V{sub H}Ox-1 gene is 99% similar to the germ-line coding sequence of the BALB/c allele. Amplification of cDNA made from splenic RNA from C57BL/6 mice confirmed that this gene is expressed. There are four nucleotide differences that lead to three amino acid changes in the predicted protein sequence. Each change is either in or immediately adjacent to a complementarity-determining region (CDR). Two of these changes are unique to the C57BL/6 allele and are not shared with CRI{sub Xmp-1}-expressing strains. These two changes are predicted to alter the Xmp binding capabilities of the C57BL/6 allelic form of this V{sub H} gene, thereby explaining the absence of the Xmp-1 clonotype, which is dominant in the primary Xmp immune response of most other strains of mice. 31 refs., 2 figs., 2 tabs.
- OSTI ID:
- 160163
- Journal Information:
- Journal of Immunology, Journal Name: Journal of Immunology Journal Issue: 5 Vol. 155; ISSN JOIMA3; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
Similar Records
Nucleotide sequence of messenger RNA encoding V[sub H]DJ[sub H] and V[sub K]J[sub K] of a highly conserved idiotype-defined primary response anti-hapten antibody
MRP-1 expression levels determine strain-specific susceptibility to sodium arsenic-induced renal injury between C57BL/6 and BALB/c mice
Characterization of the CD8{sup +} T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice
Journal Article
·
Mon Dec 14 23:00:00 EST 1992
· Journal of Immunology; (United States)
·
OSTI ID:6762013
MRP-1 expression levels determine strain-specific susceptibility to sodium arsenic-induced renal injury between C57BL/6 and BALB/c mice
Journal Article
·
Mon Feb 14 23:00:00 EST 2005
· Toxicology and Applied Pharmacology
·
OSTI ID:20634855
Characterization of the CD8{sup +} T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice
Journal Article
·
Tue Aug 15 00:00:00 EDT 2006
· Virology
·
OSTI ID:20850547