Relationship of V/sub H/ and V/sub L/ genes encoding three idiotypic families of anti-p-azobenzenearsonate antibodies

Journal Article · Sat Feb 01 04:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.4.1050· OSTI ID:6413893

Robbins, P F; Rosen, E M; Haba, S; Nisonoff, A

The authors describe here an intrastrain, cross-reactive idiotype (CRI), CRI/sub D/, associated with anti-p-azobenzenearsonate antibodies of the A/J strain of mouse and distinguishable, by some but not by all of its idiotopes, from the major anti-p-azobenzenearsonate idiotype (CRI/sub A/). Molecules carrying the CRI/sub D/ idiotype have heavy chain variable-segment sequences that are identical or nearly identical to that of the germ-line-encoded heavy chain variable sequence of CRI/sub A/. Their light chain variable sequences are very similar to those present in a third idiotypic family, CRI/sub C/, that is a minor CRI in the A/J strain but a major CRI in BALB/c. This appears to represent a form of combinational diversity, in which the heavy and light chain variable region genes of two unrelated idiotypic families interact to form a third family, all involving antibodies of the same antigen-binding (anti-p-azobenzenearsonate) specificity. The D region of CRI/sub D/, in the six monoclonal representatives studied (three IgMs, three IgGs), is unusual in that is comprises a single amino acid-arginine or serine; there are eight amino acids in the D region of CRI/sub A/ antibodies. Three different heavy chain joining regions, J/sub H/1, J/sub H/2, and J/sub H/3, are utilized. A serological reagent was developed that identifies CRI/sub D/; it was used to show that the idiotype is present in relatively high concentration, comparable to that of CRI/sub A/, in anti-p-azobenzenearsonate antibodies taken soon after primary immunization. Quantification of CRI/sub D/ was carried out with radioimmunoassay. In serum taken later CRI/sub A/ greatly predominates, but CRI/sub D/ persists at low levels.

• Research Organization: Brandeis Univ., Waltham, MA
• OSTI ID: 6413893
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:4; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIBODIES
AROMATICS
ARSENAZO
ARSENIC COMPOUNDS
ARSONIC ACIDS
AZO COMPOUNDS
BENZENE
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ELECTRON CAPTURE RADIOISOTOPES
GENES
HYBRIDOMAS
HYDROCARBONS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MESSENGER-RNA
MICE
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC ARSENIC COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOLS
POLYPHENOLS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REAGENTS
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
SULFONIC ACIDS
TRACER TECHNIQUES
VERTEBRATES