Effects of argon ions on synchronized Chinese hamster cells

Journal Article · Wed Oct 01 04:00:00 EDT 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575227· OSTI ID:6758417

Raju, M R ^[1]; Bain, E; Carpenter, S G; Jett, J; Walters, R A; Howard, J; Powers-Risius, P

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1. Univ. of California, Los Alamos, NM

The results of variation in radiosensitivity within the cell cycle of cultured Chinese hamster cells (line CHO) after exposure to argon ions compared to x rays are reported. The cells were first synchronized by mitotic selection and resynchronized in the region of late G/sub 1//early S phase by treatment with hydroxyurea (10/sup -3/ M). A fixed argon-ion dose of 400 rad at the distal end of a ridge-filter-modified Bragg peak or a 150-kVp x-ray dose of 900 rad was used to expose cells at different stages in the cell cycle. The results indicate that the variation (ratio of maximum survival tominimum survival) was approximately 25-fold for x rays, whereas there were no significant variations within the cell cycle for argon ions. Complete cell-survival curves also were obtained for argon ions and x rays at the G/sub 1//S boundary and in late S phase. No significant difference in radiosensitivity for argon ions was observed between these two cell phases. The RBE for late S cells decreased rapidly with increasing dose, whereas the RBE for cells at the G/sub 1//S boundary appeared independent of dose. At a therapeutically relevant dose per fraction of 200 rad, the RBE values for argon ions for cells at the G/sub 1//S boundary and in late S phase were 1.7 and 9.8, respectively.

• OSTI ID: 6758417
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 84:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
ANIMAL CELLS
ANIMALS
ARGON IONS
BRAGG CURVE
CELL CULTURES
CELL CYCLE
CHARGED PARTICLES
DEPTH DOSE DISTRIBUTIONS
DIAGRAMS
DOSE-RESPONSE RELATIONSHIPS
DOSES
ELECTROMAGNETIC RADIATION
HAMSTERS
HYDROXY COMPOUNDS
HYDROXYUREA
IONIZING RADIATIONS
IONS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION DOSE DISTRIBUTIONS
RADIATION DOSES
RADIATIONS
RADIOSENSITIVITY
RBE
RODENTS
SPATIAL DOSE DISTRIBUTIONS
SURVIVAL CURVES
SYNCHRONOUS CULTURES
TIME DEPENDENCE
VERTEBRATES
X RADIATION