Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Effect of dietary factors on mutagenesis, metabolism, and binding to DNA of benzo(a)pyrene and benzo(a)pyrene 7,8-dihydrodiol

Thesis/Dissertation ·
OSTI ID:6757129
Ellagic acid (EA), a naturally occurring plant phenol, at concentrations of 5 to 50 {mu}g/plate, inhibited rate liver S9 protein dependent benzo(a)pyrene (B(a)P)-induced mutagenesis in Salmonella typhimurium TA 100 by 30-81% and B(a)P 7,8-dihydrodiol (DHD)-induced mutagenesis by 29 to 75%. EA did not significantly affect the metabolism of B(a)P or B(a)P 7,8-DHD as determined by high performance liquid chromatographic analysis of the organosoluble fraction and by the quantification of water-soluble conjugates. At these concentrations EA inhibited the covalent binding of ({sup 3}H) B(a)P and ({sup 3}H) B(a)P 7,8-DHD metabolites to calf thymus DNA by 5 to 42% and 27 to 64%, respectively. Formation of benzo(a)pyrene 7,8-dihydrodiol-9,10-epoxide:deoxyguanosine (BPDE:dG) adducts was inhibited by 13 to 56% for B(a)P for B(a)P and 11 to 38% for B(a)P 7,8-DHD. These results suggest that the antimutagenic effect of EA and its inhibition of B(a)P and B(a)P 7,8-DHD metabolite-binding to DNA is not due to the inhibition of S9-mediated metabolism of these compounds. The inhibitory effect may be by previously described scavenging mechanism or by a DNA-affinity binding mechanism that prevents BPDE:DNA adduct formation.
Research Organization:
Loma Linda Univ., CA (USA)
OSTI ID:
6757129
Country of Publication:
United States
Language:
English

Similar Records

Chemiluminescence assay specific for the microsomal metabolite, benzo(a)pyrene-7,8-dihydrodiol
Journal Article · Fri Dec 31 23:00:00 EST 1982 · Anal. Biochem.; (United States) · OSTI ID:5942691
Mechanism of phage phi X174 DNA inactivation by benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide
Journal Article · Mon Aug 01 00:00:00 EDT 1977 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5794676
New type of biological chemiluminescence: the microsomal chemiluminescence of benzo(a)pyrene arises from the diol epoxide product of the 7,8-dihydrodiol
Journal Article · Fri Dec 31 23:00:00 EST 1976 · Biochem. Biophys. Res. Commun.; (United States) · OSTI ID:5088847

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ANIMALS
AROMATICS
BACTERIA
BENZOPYRENE
BIOSYNTHESIS
BODY
CALVES
CATTLE
CHROMATOGRAPHY
CONDENSED AROMATICS
DIET
DIGESTIVE SYSTEM
DNA ADDUCTS
DOMESTIC ANIMALS
GLANDS
HYDROCARBONS
HYDROGEN COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LYMPHATIC SYSTEM
MAMMALS
METABOLIC ACTIVATION
METABOLITES
MICROORGANISMS
MUTAGEN SCREENING
ORGANIC COMPOUNDS
ORGANS
RATS
RODENTS
RUMINANTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
SEPARATION PROCESSES
SYNTHESIS
THYMUS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES