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Chemiluminescence assay specific for the microsomal metabolite, benzo(a)pyrene-7,8-dihydrodiol

Journal Article · · Anal. Biochem.; (United States)
The presence of trans-7,8dihydroxy7,8-dihydrobenzo(a)-pyrene (BP-7,8-dihydrodiol) in complex metabolite mixtures produced during microsomal metabolism of benzo(a)pyrene (BP) may be determined. Only the BP-7,8-dihydrodiol metabolite will produce significant chemiluminescence (CL) in the NaOCl-H/sub 2/O/sub 2/ singlet oxygen-generating system. The limiting CL sensitivity is 30 pmol in a 1-ml CL reaction mixture. CL assays for BP-7,8-dihydrodiol in microsomal reaction solutions produced results identical with those determined by calibrated high-performance liquid chromatography. 10 references, 2 figures, 1 table.
Research Organization:
Johns Hopkins Univ., Baltimore, MD
OSTI ID:
5942691
Journal Information:
Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 130; ISSN ANBCA
Country of Publication:
United States
Language:
English

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