Vectorial (transcellular) transport of potassium (/sup 86/Rb+) by cultured Sertoli cells

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Endocrinology; (United States)

OSTI ID:6756032

Muffly, K E; Hall, P F

Sertoli cells from rats aged 25 days were grown on Millipore filters (pore diameter 0.5 micron) for 7 days and were then used for determination of transport of 86Rb+ through the cells (base to apex); this procedure is referred to as measuring transcellular or vectorial transport. Sertoli cells were also used to measure apical efflux of 86Rb+ by loading the cells with the isotope to steady state and then incubating cells so that the apical surfaces were in contact with medium not containing 86Rb+, from which samples were taken. Basal efflux was measured in the same way except that the opposite surface of the cells was in contact with the medium. Cells grown on filters treated with collagen IV plus fibronectin showed transcellular transport of 86Rb+; t1/2 for equilibration across the cells was 9-12 min. The rate of transport was accelerated by addition of (Bu)2cAMP, forskolin, or FSH to the incubation medium. Half-maximal responses were seen with (Bu)2cAMP at 0.2 mM and with forskolin at 20 microM. Apical efflux (t1/2 9.8 +/- 2.1 min) was not influenced by the presence or absence of K+ in the medium nor by azide or (Bu)2cAMP. Basal efflux showed similar values for t1/2 in the presence of K+ (9.7 +/- 1.9 min) and values of 21.4 +/- 4.2 min in the absence of K+. Vectorial transport of 86Rb+ by these cells may account for the K+ gradient seen in the seminiferous tubule and appears to result from a basolateral potassium pump together with an apical membrane that is permeable to K+.

• Research Organization: Worcester Foundation for Experimental Biology, Shrewsbury, MA (USA)
• OSTI ID: 6756032
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 123:4; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ALKALI METALS
AMP
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
FSH
GLYCOSIDES
GONADOTROPINS
HORMONES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OUABAIN
PEPTIDE HORMONES
PERMEABILITY
PITUITARY HORMONES
POTASSIUM
RADIOISOTOPES
RATS
RESPONSE MODIFYING FACTORS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
STROPHANTHINS
TRACER TECHNIQUES
VERTEBRATES