Protein secretory patterns of rat Sertoli and peritubular cells are influenced by culture conditions

Journal Article · Fri Aug 01 04:00:00 EDT 1986 · Biol. Reprod.; (United States)

DOI:https://doi.org/10.1095/biolreprod35.1.239· OSTI ID:5009200

Kierszenbaum, A L; Crowell, J A; Shabanowitz, R B; DePhilip, R M; Tres, L L

An approach combining two-dimensional gel electrophoresis and autoradiography was used to correlate patterns of secretory proteins in cultures of Sertoli and peritubular cells with those observed in the incubation medium from segments of seminiferous tubules. Sertoli cells in culture and in seminiferous tubules secreted three proteins designated S70 (Mr 72,000-70,000), S45 (Mr 45,000), and S35 (Mr 35,000). Cultured Sertoli and peritubular cells and incubated seminiferous tubules secreted two proteins designated SP1 (Mr 42,000) and SP2 (Mr 50,000). SP1 and S45 have similar Mr but differ from each other in isoelectric point (pI). Cultured peritubular cells secreted a protein designated P40 (Mr 40,000) that was also seen in intact seminiferous tubules but not in seminiferous tubules lacking the peritubular cell wall. However, a large number of high-Mr proteins were observed only in the medium of cultured peritubular cells but not in the incubation medium of intact seminiferous tubules. Culture conditions influence the morphology and patterns of protein secretion of cultured peritubular cells. Peritubular cells that display a flat-stellate shape transition when placed in culture medium free of serum (with or without hormones and growth factors), accumulate various proteins in the medium that are less apparent when these cells are maintained in medium supplemented with serum. Two secretory proteins stimulated by follicle-stimulating hormone (FSH) (designated SCm1 and SCm2) previously found in the medium of cultured Sertoli cells, were also observed in the incubation medium of seminiferous tubular segments stimulated by FSH. Results of this study show that, although cultured Sertoli and peritubular cells synthesize and secrete proteins also observed in segments of incubated seminiferous tubules anther group of proteins lacks seminiferous tubular correlates.

• Research Organization: Univ. of North Carolina, Chapel Hill
• OSTI ID: 5009200
• Journal Information: Biol. Reprod.; (United States), Journal Name: Biol. Reprod.; (United States) Vol. 1; ISSN BIREB
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BODY
CARBOXYLIC ACIDS
CELL CULTURES
CULTURE MEDIA
DRUGS
ELECTROPHORESIS
GONADS
LIPOTROPIC FACTORS
MALE GENITALS
MAMMALS
METHIONINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RATS
RODENTS
SECRETION
SOMATIC CELLS
TESTES
VERTEBRATES