Content of K+ and Na+ in seminiferous tubule and rete testis fluids from Sertoli cell-enriched testes
Journal Article
·
· Biol. Reprod.; (United States)
Seminiferous tubules of rats exposed to x-irradiation before birth were subjected to micropuncture in situ at 50 days of age to obtain samples of fluid 4 h after ligation of efferent ducts. The concentrations of cations in this fluid were: potassium, 39.7 +/- 1.2 mM, and sodium, 136.3 +/- 1.2 mM (means and standard errors, n = 5). Histologic examination revealed that germ cells constitute less than 1% of the cell population within the seminiferous tubules of these rats; the remaining cells were all Sertoli cells. Sertoli cells showed efflux of 86Rb+ with t1/2 of approximately 11 min and an active ATPase in plasma membranes. These activities were similar to those of Sertoli cells from normal rats. Germ cells from normal rats showed less rapid efflux of 86Rb+ (t1/2 greater than 60 min) and less active Na+/K+ ATPase in plasma membranes. It is concluded that Sertoli cells are responsible for the high concentration of potassium in seminiferous tubule fluid and that plasma membranes of these cells contain an active K+ pump that is not inhibited by ouabain (1 mM).
- Research Organization:
- Univ. of Virginia, Charlottesville
- OSTI ID:
- 5996815
- Journal Information:
- Biol. Reprod.; (United States), Journal Name: Biol. Reprod.; (United States) Vol. 5; ISSN BIREB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ALKALI METALS
ANIMAL CELLS
ANIMALS
ATP-ASE
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL ANALYSIS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYMES
GERM CELLS
GLYCOSIDES
HYDROLASES
IONIZING RADIATIONS
IRRADIATION
KIDNEYS
MAMMALS
MATERIALS
MEMBRANES
METALS
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PHOSPHOHYDROLASES
POTASSIUM
PRENATAL EXPOSURE
PRENATAL IRRADIATION
RADIATIONS
RATS
RODENTS
SODIUM
SOMATIC CELLS
STROPHANTHINS
TUBULES
URINE
UROGENITAL SYSTEM DISEASES
VERTEBRATES
WASTES
X RADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ALKALI METALS
ANIMAL CELLS
ANIMALS
ATP-ASE
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL ANALYSIS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYMES
GERM CELLS
GLYCOSIDES
HYDROLASES
IONIZING RADIATIONS
IRRADIATION
KIDNEYS
MAMMALS
MATERIALS
MEMBRANES
METALS
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PHOSPHOHYDROLASES
POTASSIUM
PRENATAL EXPOSURE
PRENATAL IRRADIATION
RADIATIONS
RATS
RODENTS
SODIUM
SOMATIC CELLS
STROPHANTHINS
TUBULES
URINE
UROGENITAL SYSTEM DISEASES
VERTEBRATES
WASTES
X RADIATION