Effects of instilled fibrogenic particles on the clonal growth of murine pulmonary alveolar macrophages

Journal Article · Wed Jul 01 04:00:00 EDT 1992 · Environmental Health Perspectives; (United States)

DOI:https://doi.org/10.1289/ehp.9297159· OSTI ID:6755358

Oghiso, Yoichi; Yamada, Yutaka ^[1]; Shibata, Yoshimi ^[2]

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1. National Institute of Radiological Sciences, Chiba (Japan)
2. East Carolina Univ., Greenville, NC (United States)

Murine pulmonary alveolar macrophages (PAM) form macrophage colonies in vitro with colony-stimulating factors, which stimulate the clonal growth of radioresistant alveolar colony-forming cells (AL-CFC). The toxic effects of fibrogenic mineral dust particles on AL-CFC were investigated after intratracheal instillation into mice. Exposure to either crocidolite asbestos or silica (Min-u-sil) induced a significant depletion of AL-CFC as well as a decrease in PAM recovery compared to either untreated or titanium dioxide-exposed animals. Such effects were also noted with different doses (50-200[mu]g/animal) of instilled particles. The plating efficiency of AL-CFC was depleted in PAM exposed to fibrogenic particles in vitro, but not when exposed to nonfibrogenic titanium dioxide particles. These results indicate the toxic effects of fibrogenic dust particles on the clonal growth of PAM, cells which play a role in the clearance of inhaled particles from the lung and in subsequent pathologic processes. 11 refs., 2 figs., 2 tabs.

• OSTI ID: 6755358
• Journal Information: Environmental Health Perspectives; (United States), Journal Name: Environmental Health Perspectives; (United States) Vol. 97; ISSN EVHPAZ; ISSN 0091-6765
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ASBESTOS
BIOLOGICAL PATHWAYS
CHALCOGENIDES
CLEARANCE
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
EXCRETION
LUNG CLEARANCE
MACROPHAGES
MINERALS
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
SILICA
SILICON COMPOUNDS
SILICON OXIDES
SOMATIC CELLS
TOXICITY