Degradation of 1,2,3,4-tetrachlorobenzene by Pseudomonas chlororaphis RW71

Journal Article · Thu Oct 01 04:00:00 UTC 1998 · Applied and Environmental Microbiology

OSTI ID:675509

Potrawfke, T; Timmis, K N; Wittich, R M ^[1]

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1. National Research Centre for Biotechnology, Braunschweig (Germany). Div. of Microbiology

Pseudomonas chlororaphis RW71 mineralized 1,2,3,4-tetrachlorobenzene, a highly recalcitrant pollutant hitherto not known to be degraded by pure cultures, as a sole source of carbon and energy, thereby releasing stoichiometric amounts of chloride. The transient excretion of tetrachlorocatechol in the early growth phase suggests an initial attack by a dioxygenase to form the corresponding dihydrodiol which rearomatizes to the catechol. The activity of chlorocatechol 1,2-dioxygenase in crude cell extracts was found to be extraordinarily high towards 3-chlorocatechol (ratio of 2.6 compared to catechol) and other chlorocatechols, including tetrachlorocatechol, which was transformed at a low but significant rate. Further identification of tetrachloromuconic acid, 2,3,5-trichlorodienelactone, 2,3,5-trichloromaleyl acetic acid, and 2,4-dichloro-3-oxoadipic acid as their methyl esters, together with high specific enzyme activities for chlorinated substrates, implicated a functioning chlorocatechol pathway to be induced during growth.

• OSTI ID: 675509
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 10 Vol. 64; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
AEROBIC CONDITIONS
BIODEGRADATION
CHLORINATED AROMATIC HYDROCARBONS
OXYGENASES
PSEUDOMONAS
REMEDIAL ACTION