Oxidation of substituted phenols by Pseudomonas putida F1 and Pseudomonas sp. strain JS6

Journal Article · Wed Jun 01 00:00:00 EDT 1988 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6806601

Spain, J C; Gibson, D T

The biodegradation of benzene, toluene, and chlorobenzenes by Pseudomonas putida involves the initial conversion of the parent molecules to cis-dihydrodiols by dioxygenase enzyme systems. The cis-dihydrodiols are then converted to the corresponding catechols by dihydrodiol dehydrogenase enzymes. Pseudomonas sp. strain JS6 uses a similar system for growth on toluene or dichlorobenzenes. We tested the wild-type organisms and a series of mutants for their ability to transform substituted phenols after induction with toluene. When grown on toluene, both wild-type organisms converted methyl-, chloro-, and nitro-substituted phenols to the corresponding catechols. Mutant strains deficient in dihydrodiol dehydrogenase or catechol oxygenase activities also transformed the phenols. Oxidation of phenols was closely correlated with the induction and activity of the toluene dioxygenase enzyme system.

• Research Organization: Air Force Engineering and Services Lab., Tyndall Air Force Base, FL (USA)
• OSTI ID: 6806601
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 54:6; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

510200* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
ALKYLATED AROMATICS
AROMATICS
BACTERIA
BENZENE
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DECOMPOSITION
DEVELOPERS
ENZYME ACTIVITY
ENZYMES
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
HYDROXY COMPOUNDS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
PHENOLS
POLYPHENOLS
PSEUDOMONAS
PYROCATECHOL
TOLUENE