Attempts to apply affinity labeling techniques to ribulosebisphosphate carboxylase/oxygenase. [Comparison of spinach leaf and Rhodospirillum rubrum]
Studies on carboxylases/oxygenases from different species may be necessary to confirm that a residue implicated as essential is indeed an active-site component. To provide an especially stringent test case for the identification of species invariant structural features the enzymes from two phylogenetically distant species, spinach and Rhodospirillum rubrum, were compared. To date, the reactions of Br-butanone-P/sub 2/ and BrAcNHEtOP with the spinach enayme have been rather thoroughly characterized; only preliminary experiments have been completed with the R. rubrum enzyme. Both enzymes were isolated and assayed for carboxylase activity (spectrophotometrically or /sup 14/CO/sub 2/-fixation) and for oxygenase activity.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- EY-76-C-02-0016
- OSTI ID:
- 6744100
- Report Number(s):
- CONF-780592-1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL VARIABILITY
CARBOHYDRATES
CARBOXYLASE
COMPARATIVE EVALUATIONS
ENZYMES
FOOD
GENETIC VARIABILITY
ISOTOPE APPLICATIONS
KETONES
KINETICS
LEAVES
LYASES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PENTOSES
PHOSPHATES
PHOSPHORUS COMPOUNDS
REACTION KINETICS
RIBULOSE
SACCHARIDES
SPINACH
TRACER TECHNIQUES
VEGETABLES