Inactivation of ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum and spinach with the new affinity label 2-bromo-1,5-dihydroxy-3-pentanone 1,5-bisphosphate
- Univ. of Tennessee, Oak Ridge
In an attempt to identify the active-site base believed to initiate catalysis by ribulosebisphosphate carboxylase, we have synthesized 2-bromo-1, 5-dihydroxy-3-pentanone 1,5-bisphosphate, a reactive analogue of a postulated intermediate of carboxylation. Although highly unstable, this compound can be shown to inactivate the carboxylases from both Rhodospirillum rubrum and spinach rapidly and irreversibly. Inactivation follows pseudo first-order kinetics, shows rate saturation and is greatly reduced by saturating amounts of the competitive inhibitor, 2-carboxyribitol 1,5-bisphosphate. The incorporation of reagent, quantified by reducing the modified carboxylases with (/sup 3/H)NaBH/sub 4/, shows that inactivation results from the modification of approximately one residue per catalytic subunit of the Rhodospirillum rubrum enzyme and less than one residue per protomeric unit of the spinach enzyme.
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5177575
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 103:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Reaction of ribulosebisphosphate carboxylase from Rhodospirillum rubrum with the potential affinity label 3-bromo-1,4-dihydroxy-2-butanone 1,4-bisphosphate
2-Bromoacetylaminopentitol 1,5-bisphosphate as an affinity label for ribulose bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum
Related Subjects
BROMINATED ALIPHATIC HYDROCARBONS
CHEMICAL REACTIONS
CARBOXYLASE
INACTIVATION
CATALYSIS
BIOCHEMICAL REACTION KINETICS
OXYGENASES
LABELLED COMPOUNDS
PHOSPHATES
RHODOSPIRILLUM
SPINACH
TRITIUM COMPOUNDS
BACTERIA
ENZYMES
FOOD
HALOGENATED ALIPHATIC HYDROCARBONS
KINETICS
LIGASES
MICROORGANISMS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
REACTION KINETICS
VEGETABLES
550201* - Biochemistry- Tracer Techniques