Complete primary structure of ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum
Of the 14 cyanogen bromide fragments derived from Rhodospirillum rubrum ribulosebisphosphate carboxylase/oxygenase, four are too large to permit complete sequencing by direct means. These have now been digested with proteases, and the resultant peptides have been purified and sequenced, thereby providing the complete sequences of the original fragments. With the determination of these sequences, the total primary structure of the enzyme is provided. The polypeptide chain consists of 466 residues, 144 (31%) of which are identical to those at corresponding positions of the large subunit of spinach ribulosebisphosphate carboxylase/oxygenase. Despite the low overall homology, striking homology between the two species of enzyme is observed in those regions previously implicated at the catalytic and activator sites. 36 references, 6 figures, 9 tables.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6315534
- Journal Information:
- Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 232:1; ISSN ABBIA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ribulosebisphosphate carboxylase/oxygenase: active-site characterization and partial sequence determination
Reexamination of the binding site for pyridoxal 5'-phosphate in ribulosebisphosphate carboxylase/oxygenase from Rhodospirillum rubrum
Related Subjects
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
BACTERIA
BROMIDES
BROMINE COMPOUNDS
CARBOHYDRATES
CARBON-CARBON LYASES
CARBOXY-LYASES
CATALYSIS
CHEMICAL REACTIONS
CYANOGEN
DATA
ENZYMES
EXPERIMENTAL DATA
HALIDES
HALOGEN COMPOUNDS
INFORMATION
KETONES
LYASES
MICROORGANISMS
MOLECULAR STRUCTURE
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PENTOSES
PHOSPHATES
PHOSPHORUS COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
RHODOSPIRILLUM
RIBULOSE
SACCHARIDES
SYNTHESIS