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Transformation of mouse C3H/10T1/2 cells by single and fractionated doses of x-rays and fission-spectrum neutrons

Journal Article · · Cancer Res.; (United States)
OSTI ID:6739957
A mouse embryo-derived cell line, C3H/10T1/2, was used to measure the frequency of in vitro neoplastic transformation induced by 50-kVp x-rays and by fission-spectrum neutrons from the JANUS reactor at the Argonne National Laboratory. The transformation frequency after single x-ray doses rises exponentially, reaching a plateau at about 3 x 10(-3) transformant/survivor. The induction curve following single neutron doses, while qualitatively similar, initially rises more steeply and levels off at a maximum of about 6 x 10(-3) transformant/survivor. For both radiations, transformation frequency varies with changes in the number of viable cells per dish. Fractionation of a total x-ray dose of 700 rads results in an approximately 6-fold increase in survival and reduction in transformation frequency over a 16-hr interval. Fractionation of the total neutron dose of 378 rads has no effect upon cell survival, and transformation frequency declines by a factor of only about 1.7 at most over a 24-hr period.
Research Organization:
Argonne National Lab., IL
OSTI ID:
6739957
Journal Information:
Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39:1; ISSN CNREA
Country of Publication:
United States
Language:
English