Escherichia coli gene that controls sensitivity to alkylating agents
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6738369
A new type of Escherichia coli mutant which shows increased sensitivity to methyl methane sulfonate but not to uv light or to gamma rays was isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. The mutant is unable to reactivate phage lambda vir or double-stranded phi X174 DNA (replicative form) that had been treated with methyl methane sulfonate. The mutant is sensitive to other alkylating agents, such as ethyl methane sulfonate, mitomycin C, and N-methyl-N'-nitro-N-nitrosoguanidine, as well. It grows normally and exhibits almost normal recombination proficiency. The mutant possesses normal levels of DNA polymerase I, exonuclease I, exonuclease V, endonuclease specific for methyl methane sulfonate-treated DNA, and 3-methyladenine-DNA glycosidase activities. The genetic locus responsible has been named alk and is located near his on the chromosome.
- Research Organization:
- Kyushu Univ., Fukuoka
- OSTI ID:
- 6738369
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 135:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BACTERIA
BIOLOGICAL EFFECTS
DRUGS
ELECTROMAGNETIC RADIATION
EMS
ENZYMES
ESCHERICHIA COLI
ESTERS
GAMMA RADIATION
GENETIC MAPPING
IONIZING RADIATIONS
MICROORGANISMS
MITOMYCIN
MUTAGENS
MUTANTS
NUCLEASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHOTRANSFERASES
POLYMERASES
RADIATIONS
RADIOSENSITIVITY
SENSITIVITY
SULFONIC ACID ESTERS
TRANSFERASES
ULTRAVIOLET RADIATION
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
560302 -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
BACTERIA
BIOLOGICAL EFFECTS
DRUGS
ELECTROMAGNETIC RADIATION
EMS
ENZYMES
ESCHERICHIA COLI
ESTERS
GAMMA RADIATION
GENETIC MAPPING
IONIZING RADIATIONS
MICROORGANISMS
MITOMYCIN
MUTAGENS
MUTANTS
NUCLEASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHOTRANSFERASES
POLYMERASES
RADIATIONS
RADIOSENSITIVITY
SENSITIVITY
SULFONIC ACID ESTERS
TRANSFERASES
ULTRAVIOLET RADIATION