Isolation and characterization of a UV-sensitive mutator (mutB1) mutant of Haemophilus influenzae

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · J. Bacteriol.; (United States)

OSTI ID:6922775

Walter, R B; Stuy, J H

The mutB1 mutant of Haemophilus influenzae is very sensitive to UV radiation but only slightly sensitive to methylmethane sulfonate or N-methyl-N'-nitro-N-nitrosoguanidine. Cultures of mutB1 cells contain high numbers of spontaneous mutants and show hypermutability after exposure to the latter mutagen. Normally high-efficiency transforming markers, as well as low-efficiency ones, transform mutB1 recipients at similarly low efficiencies. Significant host cell reactivation was observed when mutB1 cells were exposed to UV-damaged phage; however, these mutants showed a decrease in phage recombination. This mutant did not degrade its DNA following exposure to UV. It is speculated that the mutB1 mutation is similar to the Escherichia coli uvrD mutation.

• Research Organization: Univ. of Texas System Cancer Center, Smithville (USA)
• OSTI ID: 6922775
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 170:6; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
ELECTROMAGNETIC RADIATION
ESTERS
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTANTS
MUTATIONS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
RADIATIONS
RADIOSENSITIVITY
SULFONIC ACID ESTERS
TOXICITY
ULTRAVIOLET RADIATION
VIRUSES