Effects of x-irradiation on a temperate bacteriophage of Haemophilus influenzae. [UV radiation]

Journal Article · Fri Apr 01 04:00:00 EST 1977 · J. Virol.; (United States)

OSTI ID:7212848

Boling, M E; Randolph, M L

The inactivation of bacteriophage HPlcl by x rays in a complex medium was found to be exponential, with a D/sub 0/ (the x-ray exposure necessary to reduce the survival of the phage to 37 percent) of approximately 90 kR. Analysis of results of sucrose sedimentation of DNA from x-irradiated whole phage showed that the D/sub 0/ for intactness of single strands was about 105 kR, and for intactness of double strands, it was much higher. The D/sub 0/ for attachment of x-irradiated phage to the host was roughly estimated as about 1,100 kR. Loss of DNA from the phage occurred and was probably due to lysis of the phage by x irradiation, but the significance of the damage is not clear. The production of single-strand breaks approaches the rate of survival loss after x irradiation. However, single-strand breaks produced by uv irradiation, in the presence of H/sub 2/O/sub 2/, equivalent to 215 kR of x rays, showed no lethal effect on the phage. Although uv-sensitive mutants of the host cell, Haemophilus influenzae, have been shown to reactivate uv-irradiated phage less than does the wild-type host cell, x-irradiated phage survive equally well on the mutants as on the wild type, a fact suggesting that other repair systems are involved in x-ray repair.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 7212848
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 22:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DNA
ELECTROMAGNETIC RADIATION
HAEMOPHILUS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
INACTIVATION
IONIZING RADIATIONS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PEROXIDES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
STRAND BREAKS
ULTRAVIOLET RADIATION
VIRUSES
X RADIATION