Characterization and genetic mapping of a mutation affecting apurinic endonuclease activity in Staphylococcus aureus

Journal Article · Sat Nov 01 04:00:00 EST 1986 · J. Bacteriol.; (United States)

OSTI ID:6774992

Tam, J E; Pattee, P A

Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. Transformation analyses identified a specific mutation, designated ngr-374, that was responsible not only for N-methyl-N'-nitro-N-nitrosoguanidine sensitivity, but also sensitivity to methyl methanesulfonate, ethyl methanesulfonate, nitrous acid, and UV irradiation. However, ngr-374-carrying recombinants showed no significant increase in their sensitivity to mitomycin C or 4-nitroquinoline 1-oxide and were unaffected in recombination proficiency. In vitro assays showed that ngr-374-carrying strains had lower apurinic/apyrimidinic endonuclease activities than the wild type. The chromosomal locus occupied by ngr-374 was shown to exist in the gene order omega(Chr::Tn551)40-ngr-374-thrB106.

• Research Organization: Iowa State Univ., Ames
• OSTI ID: 6774992
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA REPAIR
DNA-ASE
ELECTROMAGNETIC RADIATION
EMS
ENDONUCLEASES
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
GENETIC EFFECTS
GENETIC MAPPING
HYDROGEN COMPOUNDS
HYDROLASES
INORGANIC ACIDS
MAPPING
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
MUTANTS
NITROSO COMPOUNDS
NITROUS ACID
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHODIESTERASES
RADIATIONS
RECOVERY
REPAIR
SENSITIVITY
STAPHYLOCOCCUS
SULFONIC ACID ESTERS
ULTRAVIOLET RADIATION