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Affinities and densities of high-affinity (/sup 3/H)muscimol (GABA-A) binding sites and of central benzodiazepine receptors are unchanged in autopsied brain tissue from cirrhotic patients with hepatic encephalopathy

Journal Article · · Hepatol.; (United States)
; ; ;
The integrity of GABA-A receptors and of central benzodiazepine receptors was evaluated in membrane preparations from prefrontal cortex and caudate nuclei obtained at autopsy from nine cirrhotic patients who died in hepatic coma and an equal number of age-matched control subjects. Histopathological studies revealed Alzheimer Type II astrocytosis in all cases in the cirrhotic group; controls were free from neurological, psychiatric or hepatic diseases. Binding to GABA-A receptors was studied using (/sup 3/H)muscimol as radioligand. The integrity of central benzodiazepine receptors was evaluated using (/sup 3/H)flunitrazepam and (/sup 3/H)Ro15-1788. Data from saturation binding assays was analyzed by Scatchard plot. No modifications of either affinities (Kd) or densities (Bmax) of (/sup 3/H)muscimol of central benzodiazepine binding sites were observed. These findings do not support recent suggestions that alterations of either high-affinity GABA or benzodiazepine receptors play a significant role in the pathogenesis of hepatic encephalopathy.
Research Organization:
Univ. of Montreal, Quebec (Canada)
OSTI ID:
6736454
Journal Information:
Hepatol.; (United States), Journal Name: Hepatol.; (United States) Vol. 8:5; ISSN HPTLD
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
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59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINO ACIDS
AMINOBUTYRIC ACID
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTOPSY
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM DISEASES
DISEASES
DRUGS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIGANDS
LIVER CIRRHOSIS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PATIENTS
PROTEINS
PSYCHOTROPIC DRUGS
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
TRANQUILIZERS
TRITIUM COMPOUNDS